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RNA-seq of MIN6 cells overexpressing Hnf1a through CRISPR activation

ABSTRACT: RNA-seq of MIN6 cells overexpressing Hnf1a through CRISPR activation

PROVIDER: PRJEB51149 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR8778760_1.fastq.gz	Fastqsanger.gz
	ERR8778760_2.fastq.gz	Fastqsanger.gz
	ERR8778770.fastq.gz	Fastqsanger.gz
	ERR8778780.fastq.gz	Fastqsanger.gz
	ERR8778761.fastq.gz	Fastqsanger.gz

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RNA-seq of MIN6 cells overexpressing Hnf1a through CRISPR activation

Project description:To determine the impact of Hnf1a overexpression on gene expression in mouse beta cells, we performed RNA-seq experiments on MIN6 where Hnf1a was overexpressed using CRISPR activation (SAM system).

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Project description:CRISPR-Cas9 was used to generate HNF1a mutants (+/- and -/-) in human embryonic stem cell lines and used an in vitro differentiation system to study pancreatic beta cell differentiation and subsequent function of the derivative beta cells.

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UMI-4C in control and HASTER-deficient EndoC-betaH3 cells overexpressing HNF1A

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