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The Gut Microbiome Among Postmenopausal Latvian Women: The Influence of Dietary Habits

ABSTRACT: Gut microbiome in postmenopausal women, yoghurt experiment

PROVIDER: PRJEB52634 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR9744470_1.fastq.gz	Fastqsanger.gz
	ERR9744470_2.fastq.gz	Fastqsanger.gz
	ERR9744480_1.fastq.gz	Fastqsanger.gz
	ERR9744480_2.fastq.gz	Fastqsanger.gz
	ERR9744490_1.fastq.gz	Fastqsanger.gz

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Project description:In recent years, many studies have been initiated to characterise the human gut microbiome in relation to different factors like age, lifestyle, and dietary habits. This study aimed to evaluate the impact of yoghurt intake on the gut microbiome among postmenopausal women and how overall dietary habits modulate the gut microbiome. In total, 52 participants were included in the study and two groups-a control (n = 26) and experimental group (n = 26)-were established. The study was eight weeks long. Both study groups were allowed to consume a self-selected diet, but the experimental group had to additionally consume 175 g of plain organic milk yoghurt on a daily basis for eight weeks. In addition, a series of questionnaires were completed, including a questionnaire on the subject's sociodemographic background, health status, and lifestyle factors, as well as a food frequency questionnaire. Stool samples were collected for the analysis of the gut microbiome (both prior to and after the eight weeks of the study). Sequencing of V4-V5 regions of the 16S rRNA gene was used to determine the bacterial composition of stool samples. The dominant phylum from the gut microbiome was Firmicutes (~70% to 73%), followed by Bacteroidota (~20% to 23%). Although no significant changes in the gut microbiome were related to daily consumption of yoghurt, we report that consumption of food products like grains, grain-based products, milk and milk products, and beverages (tea, coffee) is associated with differences in the composition of the gut microbiome. Establishing nutritional strategies to shape the gut microbiome could contribute to improved health status in postmenopausal women, but further research is needed.

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