Project description:Phytogenic feed additives offer health benefits, improved overall performance and well-being in poultry production and provide an alternative to in-feed antibiotics. In this experiment, post-peak laying hens were fed for a period of 4 weeks either Control or Supplemental diet, a phytogenic feed additive based with essential oils from thyme (Thymus vulgaris) and star anis (Illicium verum) as well as Quillaja bark (Quillaja Saponaria). RNA sequencing was performed in the pancreas, kidney, uterus and large-white follicles of 10 birds from each dietary groups at the end of the trial.
Project description:Profile the expression of micoRNAs in the human brain at prenatal, early childhood, and adult timepoints 37 prenatal cerebral tissue samples, 11 postnatal cerebral tissue samples, 2 adult total brain samples (FirstChoice Human Brain Reference RNA cat# AM6050 and FirstChoice Human Brain Total RNA cat# AM7962*. *The current FirstChoice Human Brain Total RNA (2013), is from a 78 year old white female, while the FirstChoice Total RNA used in this deposited data set was from an 81 year old white male, which is no longer available through Ambion/Life Technologies.)
Project description:We report differential expressed genes in white and brown adipose tissues with surgical denervation procedure in male mice fed with a high-fat diet.
Project description:We report differential expressed genes in white and brown adipose tissues with surgical denervation procedure in male mice fed with a low-fat diet.
Project description:Vanishing white matter (VWM) is a leukodystrophy that primarily manifests in young children. In this disease, the brain white matter is differentially affected in a predictable pattern with telencephalic brain areas being more severely affected, while others remain allegedly completely spared. Using high-resolution mass spectrometry-based proteomics, we investigated the proteome patterns of the severely affected white matter in the frontal lobe and normal appearing pons in VWM and control cases to identify molecular bases underlying regional vulnerability. By comparing VWM patients to controls, we identified disease-specific proteome patterns. We showed substantial pathogenic changes in both the frontal white matter and pons at the protein level. Side-by-side comparison of brain region-specific proteome patterns further revealed regional differences. We found that different cell types are affected in the VWM frontal white matter than in the pons. Gene ontology and pathway analyses identified involvement of region distinct biological processes, of which pathways implicated in cellular respiratory metabolism were overarching features. In the VWM frontal white matter, proteome changes were associated with decrease in glycolysis/gluconeogenesis and metabolism of various amino acids. By contrast, in the VWM pons white matter, we found a decrease in oxidative phosphorylation. Taken together, our data show that brain regions are affected in parallel in VWM, but to different degrees. We found region-specific involvement of different cell types and discovered that cellular respiratory metabolism is differently affected across white matter regions in VWM. These region-specific changes help explain regional vulnerability to pathology in VWM.
Project description:Purpose: To investigate the effect that acute ethanol tretament (5 g/kg) has on the brain transcriptome and relate that to ethanol-related behaviors. Methods: Male LXS recombinant inbred mice (40 strains; n=2 or 3 per strain) were treated with saline or ethanol (i.p.; 5 g/kg). Paired-end RNA-seq methods were employed to assess mRNA expression in whole brain 8 hours after the treatment. Results: The expression of thousands of transcripts was altered by the ethanol treatment. Thousands of expression QTLs (eQTLs) were mapped. A large percentage of the eQTLs were unique to the treatment.
