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Soil-borne mycorrhiza

ABSTRACT: Mycorrhizal fungi under contrasting fertilization strategies

PROVIDER: PRJEB53717 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR9874790_1.fastq.gz	Fastqsanger.gz
	ERR9874790_2.fastq.gz	Fastqsanger.gz
	ERR9874900_1.fastq.gz	Fastqsanger.gz
	ERR9874900_2.fastq.gz	Fastqsanger.gz
	ERR9874791_1.fastq.gz	Fastqsanger.gz

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Mycorrhiza

Project description:arbuscular mycorrhizal fungi

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A roadmap of cell-type specific gene expression during late stages of the arbuscular mycorrhiza symbiosis

Project description:Arbuscular mycorrhiza (AM) interactions between plants and Glomeromycota fungi primarily support phosphate aquisition of most terrestrial plant species. To unravel cell-type specific gene expression during late stages of Medicago truncatula root colonization by AM fungi, we used genome-wide transcriptome profiling based on laser-microdissected cells. We used Medicago GeneChips to detail the cell-type specific programme of gene expression in late stages of colonization by arbuscular mycorrhizal fungi and identified genes differentially expressed during these stages. Medicago truncatula Gaertn M-bM-^@M-^XJemalongM-bM-^@M-^Y genotype A17 plantlets were grown in the climate chamber. Plants grown for the collection of root cortical cells containing arbuscules (ARB), root cortical cells from mycorrhizal roots (CMR), and root epidermal cells from mycorrhizal roots (EPI) were mycorrhized after 2 weeks with Glomus intraradices and mycorrhizal roots were harvested at around 21 days post inoculation (dpi).

2013-04-28 | E-GEOD-42747 | biostudies-arrayexpress

Responsivity of two pea genotypes to the symbiosis with rhizobia and arbuscular mycorrhiza fungi – a proteomics aspect of the “Efficiency of Interactions with Beneficial Soil Microoganisms” trait

Project description:It is well-known that individual pea (Pisum sativum L.) cultivars differ in their symbiotic responsivity. This trait is typically manifested with an increase in seed weights due to inoculation with rhizobial bacteria and arbuscular mycorrhizal fungi. The aim of this work was to characterize the alterations in root proteome of highly responsive pea genotype k-8274 and low-responsive genotype k-3358 grown in non-sterile soil, which were associated with root colonization with rhizobial bacteria and arbuscular mycorrhiza fungi in comparison to proteome shifts caused by soil supplementation with mineral nitrogen salts. Our results clearly indicate that supplementation of the soil with mineral nitrogen-containing salts switched the root proteome of both genotypes to assimilation of the available nitrogen, whereas the processes associated with nitrogen fixation were suppressed. Surprisingly, inoculation with rhizobial bacteria had only a minor effect on root proteomes of the both genotypes. The most pronounced response was observed for highly responsive k-8274 genotype inoculated simultaneously with rhizobial bacteria and arbuscular mycorrhizal fungi. This response involved activation of the proteins related to redox metabolism and suppression of excessive nodule formation. In turn, the low-responsive genotype k-3358 demonstrated a pronounced inoculation-induced suppression of protein metabolism and enhanced diverse defense reactions in pea roots under the same soil conditions. The results of the study shed light on the molecular basis of differential symbiotic responsivity in different pea cultivars.

2025-02-24 | PXD058701 | Pride

A roadmap of cell-type specific gene expression during late stages of the arbuscular mycorrhiza symbiosis

2013-04-28 | GSE42747 | GEO

A roadmap of cell-type specific gene expression during early stages of the arbuscular mycorrhiza symbiosis

2013-04-28 | GSE42746 | GEO

Effect of fertilization strategies on soil 16S bacterial communities

Project description:Characterization of bacterial communities in soil under no-tillage practices with contrasting fertilization strategies

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Gene expression data from arbuscular mycorrhizal and phosphate-treated Medicago truncatula roots.

Project description:Arbuscular mycorrhiza (AM) interactions between plants and Glomeromycota fungi primarily support phosphate aquisition of most terrestrial plant species. To unravel gene expression in Medicago truncatula root colonization by AM fungi, we used genome-wide transcriptome profiling based on whole mycorrhizal roots. We used GeneChips to detail the global programme of gene expression in response to colonization by arbuscular mycorrhizal fungi and in response to a treatment with phosphate and identified genes differentially expressed during this process. Medicago truncatula roots were harvested at 28 days post inoculation with the two different arbuscular mycorrhizal fungi Glomus intraradices (Gi-Myc) and Glomus mosseae (Gm-Myc) under low phosphate conditions (20 µM phosphate) or after a 28 days treatment with 2 mM phosphate in the absence of arbuscular mycorrhizal fungi (2mM-P). As a control, uninfected roots grown under low phosphate conditions (20 µM phosphate) were used (20miM-P). Three biological replicates consisting of pools of five roots were used for RNA extraction and hybridization on Affymetrix GeneChips.

2011-10-28 | E-GEOD-32208 | biostudies-arrayexpress

A roadmap of cell-type specific gene expression during early stages of the arbuscular mycorrhiza symbiosis

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Mildew locus O facilitates colonization by arbuscular mycorrhiza in angiosperms

Project description:RNA-sequencing of Hordeum vulgare cv. Ingrid wild type and Hvmlo1-5 mutant in non-mycorrhizal (control) and mycorrhizal roots at 17 days post inoculation (dpi) and 26 dpi.

2020-01-25 | GSE144199 | GEO

soil metagenome

Project description:Arbuscular Mycorrhizal Fungi Metagenome

| PRJNA239364 | ENA