Project description:Using 5' droplet-based single cell sequencing, we profiled single cells dervied from human colorectal cancer organoids carrying either APC mutation or RSPO fusion, and paired normal colon organoids for the later.
Project description:To identify transcription factors important for salivary gland organoids and associated to salivary gland stem/progenitor cells, we performed ATAC-seq of organoids derived from non-irradiated mice of different ages.
Project description:Patient-derived colorectal cancer organoids were injected into mice to grow into subcutaneous tumors. Tumors were profiled through multiplexed single-cell RNA-sequencing.
Project description:We performed quantitative mass spectrometry based proteomic analysis and comparative transcriptomics analysis of 7 individual patient-derived human colorectal tumor and healthy organoids, the latter acquired from adjacent healthy tissue.
Project description:We report the first use of genome-edited human kidney organoids, combined with single-cell transcriptomics, to study APOL1 risk variants at the native genomic locus in different nephron cell types. This approach captures interferon-mediated induction of APOL1 gene expression and cellular dedifferentiation with a secondary insult“second hit” of endoplasmic reticulum stress.