Feed your microbes to deal with stress: a psychobiotic diet impacts microbial stability and perceived stress in a healthy adult population
Ontology highlight
ABSTRACT:
PROVIDER: PRJEB56264 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| ERR10233700_1.fastq.gz | Fastqsanger.gz | |||
| ERR10233700_2.fastq.gz | Fastqsanger.gz | |||
| ERR10233800_1.fastq.gz | Fastqsanger.gz | |||
| ERR10233800_2.fastq.gz | Fastqsanger.gz | |||
| ERR10233900_1.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 5 of 760 |
Similar Datasets
Project description:The impact of diet on the microbiota composition and the role of diet in supporting optimal mental health have received much attention in the last decade. However, whether whole dietary approaches can exert psychobiotic effects is largely understudied. Thus, we investigated the influence of a psychobiotic diet (high in prebiotic and fermented foods) on the microbial profile and function as well as on mental health outcomes in a healthy human population. Forty-five adults were randomized into either a psychobiotic (n = 24) or control (n = 21) diet for 4 weeks. Fecal microbiota composition and function was characterized using shotgun sequencing. Stress, overall health and diet were assessed using validated questionnaires. Metabolic profiling of plasma, urine and fecal samples was performed. Intervention with a psychobiotic diet resulted in reductions of perceived stress (32% in diet vs. 17% in control group), but not between groups. Similarly, biological marker of stress were not affected. Additionally, higher adherence to the diet resulted in stronger decreases in perceived stress. While the dietary intervention elicited only subtle changes in microbial composition and function, significant changes in the level of 40 specific fecal lipids and urinary tryptophan metabolites were observed. Lastly, microbial volatility was linked to greater changes in perceived stress scores in those on the psychobiotic diet. These results highlight that dietary approaches can be used to reduce perceived stress in a human cohort. Using microbiota-targeted diets to positively modulate gut-brain communication holds possibilities for the reduction of stress and stress-associated disorders, but additional research is warranted to investigate underlying mechanisms, including the role of the microbiota.
| S-EPMC9908549 | biostudies-literature
Project description:The influence of dietary factors on brain health and mental function is becoming increasingly recognized. Similarly, mounting evidence supports a role for gut microbiota in modulating central nervous system function and behaviour. Still, the molecular mechanisms responsible for the impact of diet and associated microbiome in adult neurodegeneration are still largely unclear. In this study, we aimed to investigate whether and how changes in diet-associated microbiome and its metabolites impact on adult neurogenesis. Mice were fed a high-fat, choline-deficient diet, developing obesity and several features of the metabolic syndrome, including non-alcoholic steatohepatitis. Strikingly, our results showed, for the first time, that animals fed with this specific diet display premature increased neurogenesis, possibly exhausting the available neural stem cell pool for long-term neurogenesis processes. The high-fat, choline-deficient diet further induced neuroinflammation, oxidative stress, synaptic loss and cell death in different regions of the brain. Notably, this diet-favoured gut dysbiosis in the small intestine and cecum, up-regulating metabolic pathways of short-chain fatty acids, such as propionate and butyrate and significantly increasing propionate levels in the liver. By dissecting the effect of these two specific short-chain fatty acids in vitro, we were able to show that propionate and butyrate enhance mitochondrial biogenesis and promote early neurogenic differentiation of neural stem cells through reactive oxygen species- and extracellular signal-regulated kinases 1/2-dependent mechanism. More importantly, neurogenic niches of high-fat, choline-deficient-fed mice showed increased expression of mitochondrial biogenesis markers, and decreased mitochondrial reactive oxygen species scavengers, corroborating the involvement of this mitochondrial stress-dependent pathway in mediating changes of adult neurogenesis by diet. Altogether, our results highlight a mitochondria-dependent pathway as a novel mediator of the gut microbiota-brain axis upon dietary influences.
| S-EPMC7780462 | biostudies-literature
Project description:Feed your microbes to deal with stress: a psychobiotic diet impacts microbial stability and perceived stress in a healthy adult population
| PRJEB56218 | ENA
Project description:In utero and early neonatal exposure to maternal stress is linked with psychiatric disorders, and the underlying mechanisms are currently being elucidated. We used a prenatal stressor in pregnant mice to examine novel relationships between prenatal stress exposure, changes in the gut microbiome, and social behavior. Here, we show that males exposed to prenatal stress had a significant reduction in social behavior in adulthood, with increased corticosterone release following social interaction. Male offspring exposed to prenatal stress also had neuroinflammation, decreased oxytocin receptor, and decreased serotonin metabolism in their cortex in adulthood, which are linked to decreased social behavior. Finally, we found a significant difference in commensal microbes, including decreases in Bacteroides and Parabacteroides, in adult male offspring exposed to prenatal stress when compared to non-stressed controls. Our findings indicate that gestation is a critical window where maternal stress contributes to the development of aberrant social behaviors and alterations in cortical neurobiology, and that prenatal stress is sufficient to disrupt the male gut-brain axis into adulthood.
| S-EPMC6542272 | biostudies-literature
Project description:(1) Introduction: The global rise of gastrointestinal diseases, including colorectal cancer and inflammatory bowel diseases, highlights the need to understand their causes. Diet is a common risk factor and a crucial regulator of gene expression, with alterations observed in both conditions. This study aims to elucidate the specific biological mechanisms through which diet influences the risk of bowel diseases. (2) Methods: We analyzed data from 436 participants from the BarcUVa-Seq population-based cross-sectional study utilizing gene expression profiles (RNA-Seq) from frozen colonic mucosal biopsies and dietary information from a semi-quantitative food frequency questionnaire. Dietary variables were evaluated based on two dietary patterns and as individual variables. Differential expression gene (DEG) analysis was performed for each dietary factor using edgeR. Protein-protein interaction (PPI) analysis was conducted with STRINGdb v11 for food groups with more than 10 statistically significant DEGs, followed by Reactome-based enrichment analysis for the resulting networks. (3) Results: Our findings reveal that food intake, specifically the consumption of blue fish, alcohol, and potatoes, significantly influences gene expression in the colon of individuals without tumor pathology, particularly in pathways related to DNA repair, immune system function, and protein glycosylation. (4) Discussion: These results demonstrate how these dietary components may influence human metabolic processes and affect the risk of bowel diseases.
| S-EPMC11434945 | biostudies-literature
Project description:Background and objectiveCharacterizing nicotine pharmacokinetics is challenging in the presence of background exposure. We performed a combined retrospective population pharmacokinetic analysis of 8 trials, including exposure to Tobacco Heating System and cigarettes (both inhaled), nicotine nasal spray and oral nicotine gum.MethodData from 4 single product use trials were used to develop a population pharmacokinetic model with Phoenix® NLME™ and to derive exposure parameters. Data from 4 separate ad libitum use studies were used for external validation. A total of 702 healthy adult smokers (54% males; 21-66 years of age; smoking ≥10 cigarettes/day; from US, Europe and Japan) were eligible for participation.ResultsTwo-compartment linear disposition combined with zero-order absorption model was adequate to describe nicotine pharmacokinetics, and a mono-exponentially decreasing background component was utilized to account for nicotine carry-over effects. Apparent nicotine clearance was typically 0.407 L/min in males and 26% higher in females (68% inter-individual variability). Bioavailability was product-specific, decreased with increasing nicotine ISO yield, and increased with increasing body weight. Absorption duration was apparently prolonged with nicotine gum. The typical initial and terminal half-lives were 1.35 and 17 h, respectively. The presence of menthol did not impact the determinants of the area under the curve. The model adequately described the external validation data.ConclusionsThe population model was able to describe in different populations the nicotine pharmacokinetics after single product use and after 4 days of ad libitum use of Tobacco Heating System, cigarettes, and of different nicotine replacement therapies with various routes of administration.
| S-EPMC5681983 | biostudies-literature
Project description:BackgroundSustainable nutrition is based on foods with a low environmental impact, accessible and affordable, ensuring protection of the biodiversity, while including the cultural elements of each geographical region. The present cross-sectional study aimed to evaluate adherence to a sustainable diet and the perceived environmental benefit of adopting a sustainable diet among adults in Greece.MethodsThe Sustainable HEalthy Diet (SHED) questionnaire evaluated sustainable nutrition practices and awareness in a sample of 607 adult Greeks recruited through social media.ResultsA positive association was revealed between healthy eating, a plant-based diet and organic awareness. Healthy eating was related to the consumption of low-salt and low-sugar products, avoiding added salt and ultra-processed foods (UPFs), as well as limiting sweets and soft drinks. Most participants (94.6%) were flexitarians, consuming meat instead of plant-based foods, although showing a preference for legumes over meat products. Most (86.8%) failed to meet the 5-a-day recommendations for fruit and vegetables, which were bought mainly from supermarket chains, with men resolving to electronic commerce purchases, while women preferring small, local grocery shops. Most responders consumed tap water (54.9%) and homemade meals daily (75.0%). Many participants (32.8%) reported separating and recycling food scraps at home, using neighborhood composters. When organic produce was selected, this involved mainly fruits and vegetables. Between men and women, the latter adopted a plant-based diet to a greater extent, consumed fewer soft drinks, were keener to consume local produce, limit meat intake and eat crops that are pesticide- and herbicide-free. Overall, Greek consumers show preference to local products. Most of them fail to compost and cut down on meat intake. The tool's internal consistency measured by Cronbach alpha was 0.702 and 0.736 for the healthy eating and sustainable eating domains respectively, 0.798 for the environmental domain, while the other domains had lower scores due to contradictory questions. Our Confirmatory Factor Analysis (CFA) demonstrated a good fit (CFI = 0.896, TLI = 0.87) with strong positive relationships between healthy eating, a plant-based diet and organic awareness.ConclusionsThe results indicate that adult Greeks more easily implement some aspects of sustainable nutrition (organic, local foods), while others appear more difficult (compost, reduce meat intake). Nonetheless, the results can be useful in designing interventions to increase dietary sustainability awareness among Greeks, including educational programs and improved infrastructures.
| S-EPMC11863825 | biostudies-literature
Project description:This data article reports the values of indicators of bovine health determined in the plasma of Normand cull-cows at different times of the about 100 days lasting finishing period and at slaughter. The data constitute a large dataset based on the quantification of metabolites and the evaluation of enzymes activities allowing the determination of antioxidant capacity, oxidative stress level, energy and lipid metabolisms, activity of the Hypothalamo-Pituitary-Adrenal axis and the hepatic status in cull-cows.
| S-EPMC6288407 | biostudies-literature
Project description:BackgroundDifferent food price sources and dietary assessment tools may impact the estimation of diet costs and hamper our understanding of the relationship between diet costs and dietary intakes.ObjectivesWe aimed to investigate the effect of 3 diet cost derivation methods, with increasing numbers of food prices and geographic specificity, holding consistent the dietary assessment tool, on the estimation of diet costs overall and by food group.MethodsWe matched 24-h dietary recall data from the 2015 Canadian Community Health Survey-Nutrition (CCHS-N) to food price data from 3 Canadian Consumer Price Index (CPI) food price lists; national short list, national long list, and provincial long list. We compared the daily ($/day) and energy-adjusted ($/2000 kcal) diet costs overall and by food groups for the overall population (4+), children (4-18), and adults (19+).ResultsThe proportion of dietary intakes (grams) that were covered by CPI prices significantly increased from the national short list to the national long list but did not significantly differ from the national long list to the provincial long list. The national short list resulted in the highest daily and energy-adjusted diet costs overall. No difference in diet costs was noted between the national and provincial long lists. Diet costs for 4 food groups-additions, sweets, fruits, and vegetables, which were poorly covered by the national short list-significantly differed using the national and provincial long lists. All 3 diet cost methods were significantly correlated with energy intakes; however, a strong/very strong correlation was detected for children, and a weak/moderate correlation for adults.ConclusionsThe choice of food price data may introduce bias in the diet cost estimate, as well as limiting our understanding of how individuals allocate their diet costs. Refinement of diet cost estimation methodology and measures can strengthen future studies of how consumers allocate their purchases to their diets.
| S-EPMC11600118 | biostudies-literature
Project description:Long-term effects of timber harvesting on hemicellulolytic microbial populations in coniferous forest soils
| PRJEB9181 | ENA