Project description:Next-generation sequencing (NGS)-derived transcriptomic profiling was carried out to compare the transcriptomic changes mediated by COMT knockout in U87 cells. We found significant enrichment of gene signatures involving IFNa/b signaling in COMT-KO U87 cells compared to control cells. Our analysis also demonstrated that COMT deficiency interferes with mitochondrial functions and induces the expression of genes associated with the anti-viral RNA sensing pathway.
Project description:To identify the different status of iNKT cells treated by aGalcer/IL-2 from CHB and HCC patients compared to HD. We enriched α-GalCer/IL-2 expanded iNKT cells from healthy donors (HD iNKT), CHB patients (CHB iNKT), and HBV-infected HCC patients (HCC iNKT) to rperforme next-generation sequencing (NGS). Illumina mRNA-seq sample preparation kit was used to perform paired-end library sequencing with Illumina HiSeq 2000 and sequence analysis was determined using the Illumina data analysis pipeline. Based on the comparison results,we performed the GO and KEGG pathway enrichment analysis. That might suggest the different status of iNKT cells treated by aGalcer/IL-2 between HD, CHB and HBV-infected HCC patients.