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Isolation and genome sequencing of Streptomyces sp. from the soil in Sudan

ABSTRACT: Isolation and genome sequencing of Streptomyces sp. from the soil in Sudan

PROVIDER: PRJEB59914 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	ERR11144737.fastq.gz	Fastqsanger.gz
	ERR11145445.fastq.gz	Fastqsanger.gz
	ERR11144646.fastq.gz	Fastqsanger.gz

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Isolation and genome sequencing of Streptomyces sp. from the soil in Sudan (part 2)

Project description:Isolation and genome sequencing of Streptomyces sp. from the soil in Sudan (part 2)

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Melatonin reverses mitochondria dysfunction and oxidative stress-induced apoptosis of Sudan I-exposed mouse oocytes Exposure to Sudan I compromises oocyte quality via the induction of mitochondrial dysfunction and oxidative stress in mice

Project description:Sudan I is one of the industry dyes and widely used in cosmetics, wax agent, solvent and textile. Sudan I has multiple toxicity such as carcinogenicity, mutagenicity, genotoxicity and oxidative damage. However, Sudan I has been illegally used as colorant in food products, triggering worldwide attention about food safety. Nevertheless, the toxicity of Sudan I on reproduction, particularly on oocyte maturation is still unclear. In the present study, using mouse in vivo models, we report the toxicity effects of Sudan I on mouse oocyte. The results reflect that Sudan I exposure disrupts spindle organization and chromosomes alignment as well as cortical actin distribution, thus leading to the failure of polar body extrusion. Based on the transcriptome results, it is found that the exposure of Sudan I leads to the change in expression of 764 genes. Moreover, it’s further reflected that the damaging effects of Sudan I are mediated by the destruction of mitochondrial functions, which induces the accumulated ROS to stimulate oxidative stress-induced apoptosis, while in vivo supplementation of melatonin efficaciously suppresses mitochondrial dysfunction and the accompanying apoptosis, thus reverses oocyte meiotic deteriorations. Collectively, our results prove the reproduction toxicity of Sudan I for the exposure of Sudan I reduces the oocyte quality, and demonstrate a feasible solution to mitigating Sudan I-induced meiotic deteriorations.

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Streptomyces secondary metaboites - strain M1

Project description:In this study, we describe the isolation and identification of Streptomyces isolates collected from traditional medicinal plants’ rhizosphere during a campaign in Hamedan Province, Iran. Traditional medicinal plants represent a rich and unique source for the isolation of Streptomyces and new antimicrobial compounds. This strain was isolated from the rhizosphere of Helichrysum rubicundum

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