Project description:We used a new technology, named Detect-seq, to perform genome sequencing on transfected HEK293T cells to see DdCBEs' off-target mutations. Besides, targeted-amplicon sequencing and ATAC-seq data were applied to validate the results of Detect-seq.
Project description:Periodontal pathogens (Porphyromonas gingivalis, Fusobacterium nucleatum) are known as one of several important bacterial pathogens associated with sporadic Alzheimer's disease (AD). It is suggested that the entry of Periodontal pathogens into the brain is due to the disruption of the tight junctions of endothelial cells by Periodontal pathogens. However, genomic alterations that occur as a result of Periodontal pathogens infection in endothelial cells are not well understood. Our goal was to identify genes associated with Periodontal pathogens infection-induced endothelial dysfunction by integrating gene expression data.
Project description:An Easy Operating Pathogen Microarray (EOPM) was designed to detect almost all known pathogens and related species based on their genomic sequences. For effective identification of pathogens from EOPM data, a statistical enrichment algorithm has been proposed and further implemented in a user-friendly interface.
Project description:We used a new technology, named Detect-seq, to perform genome sequencing on transfected HEK293T cells to see DdCBEs' off-target mutations. Besides, targeted-amplicon sequencing and ATAC-seq data were applied to validate the results of Detect-seq.
Project description:We used a new technology, named Detect-seq, to perform genome sequencing on transfected HEK293T cells to see DdCBEs' off-target mutations. Besides, targeted-amplicon sequencing, ATAC-seq and in situ ChIP-seq data were applied to validate the results of Detect-seq.