Project description:Senegalese sole (Solea senegalensis) were treated with CuSO4 or lipopolysaccharide (LPS), an immune-system stimulant. Controls were treated with phosphate-buffered saline (PBS). Fish were sampled after 6 hours or 24 hours. Hepatic RNA from each treatment group was combined into pools, reverse transcribed to cDNA and labeled with Cy3-dCTP or Cy5-dCTP. Hybridisations consisted of a labeled pooled sample versus a reference mixture made from all PBS-treated samples. The microarray used was made from amplicons of European flounder (Platichthys flesus) hepatic cDNA.
Project description:A Platichthys flesus cDNA microarray (GENIPOL) comprised of 12,700 clones, was used to determine and characterize gene expression profiles of flounders (Platichthys flesus) exposed to polybrominated diphenyl ethers (pentamix).
Project description:Using the GENIPOL flounder cDNA microarray, we assessed the temporal transcriptomic responses of Platichthys flesus to model toxicants over a 16 day timespan. Immature fish were treated by intraperitoneal injection with cadmium chloride (50 micrograms/kg in saline), 3-methylcholanthrene (25mg/kg in olive oil), Aroclor 1254 (50mg/kg in olive oil), tert-butyl-hydroperoxide (5mg/kg in saline), lindane (25 mg/kg in olive oil), perfluoro-octanoic acid (100mg/kg in olive oil), olive oil or saline (0.9%). Hepatic gene expression changes were determined 1,2,4,8 and 16 days post-injection in comparison with time-matched carrier controls.
Project description:Proteomics of Platichthys flesus livers after sampling different estuarine sites to describe key proteins in response to environmental stresses.
