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ATAC-seq in naive B cells, CD11c+T-bet+ B cells, CD11c-T-bet- B cells from Bm12-induced lupus T-bet reporter mice

ABSTRACT: ATAC-seq in naive B cells, CD11c+T-bet+ B cells, CD11c-T-bet- B cells from Bm12-induced lupus T-bet reporter mice

PROVIDER: PRJEB62189 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR11449389_1.fastq.gz	Fastqsanger.gz
	ERR11449389_2.fastq.gz	Fastqsanger.gz
	ERR11449390_1.fastq.gz	Fastqsanger.gz
	ERR11449390_2.fastq.gz	Fastqsanger.gz
	ERR11449391_1.fastq.gz	Fastqsanger.gz

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RNA-seq data of ABC from Bm12-induced lupus model

Project description:Age-associated B cells (ABCs) accumulate in systemic autoimmunity, and contributes to pathogenesis. ABCs are characterized by high expression of CD11c and T-bet. As a transcription factor, T-bet cannot be labeled in live cells with antibodies, so we constructed a reporter mouse strain in which fluorescent protein tdTomato fused to T-bet. Then we sorted ABCs (CD11c+T-bet+CD21- B cell) and non-ABCs (CD11c-T- bet- B cell) from the Bm12-induced lupus model for RNA sequencing.

2023-02-16 | E-MTAB-12568 | biostudies-arrayexpress

CD19+CD11c+T-bet+ B cells in myasthenia gravis: A potential biomarker

Project description:Background: Myasthenia gravis (MG), an autoimmune disorder characterized by B cell-driven autoantibody production, exhibits heterogeneous B cell subsets dysregulation and incompletely defined signaling mechanisms. Methods: A cohort of 20 naïve MG patients positive for anti-acetylcholine receptor (AChR) antibodies and 15 healthy controls was analyzed. Peripheral blood mononuclear cells underwent proteomic profiling, flow cytometry (age-associated B cells (ABCs), plasma cells, T follicular helper cells, and regulatory B cells), and western blot validation of nuclear factor kappa-B (NF-κB)/cellular reticuloendotheliosis oncogene homolog (c-Rel) expression. Clinical severity was assessed using quantitative MG (QMG) scores. Statistical analyses included differential protein expression, pathway enrichment, and receiver operating characteristic (ROC) curve evaluation. Results: Proteomics revealed significant activation of the B cell receptor and NF-κB/c-Rel signaling pathways in MG patients, validated by upregulated NF-κB/c-Rel expression (P < 0.01). Flow cytometry demonstrated elevated ABCs (CD19⁺CD11c⁺T-bet⁺), plasma cells, and T follicular helper cells, alongside reduced regulatory B cells in MG (P < 0.001). The proportion of ABCs correlated positively with QMG scores (r = 0.5015, P = 0.024) but not with AChR antibody titers, suggesting antibody-independent mechanisms. ROC analysis identified moderate diagnostic utility of ABCs for moderate-to-severe MG (QMG scores 6; area under the curve = 0.68, 95 % confidence intervals: 0.42 – 0.94). Conclusions: This study establishes ABCs and NF-κB/c-Rel signaling as central contributors to AChR-MG immunopathology. Therefore, ABCs may serve as complementary biomarkers for clinical stratification.

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Project description:Gene expression profiling of human CD11c+ CD19+ vs CD11C- CD19+ B-cells

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Small RNA Solexa sequencing of mouse CD11c+Ia high and CD11c+Ia low cells

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BLUEPRINT EpiVar RNA-seq for Naive naive CD4+ T-cells

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