Project description:Nestin-expressing progenitors (NEPs) of the developing cerebellum give rise to Bergmann glia, astrocytes and molecular layer interneurons via molecularly distinct subtypes. NEPs can be cultured in vitro as neurosphere in presence of growth factors. In order to characterise the primary neurosphere cultures generated from mouse and human developing cerebella, we performed single cell RNA-sequencing of primary NEP cultures established from 9-17 post-conception week human fetal cerebella and postnatal day 1 old mouse cerebella from two different mouse strains.
Project description:Human bone marrow-derived marrow stromal cell (BM-MSC) and neurosphere (BM-neurosphere) transplantation shows limited efficacy as a therapeutic adjunct to surgical decompression in an implantable polymer-induced degenerative cervical myelopathy (DCM) model Degenerative cervical myelopathy (DCM) is a prevalent spinal cord disorder in the developed world. The study investigates the therapeutic potential of human bone marrow-derived mesenchymal stem cells (BM-MSC) and BM-neurospheres as adjunct therapies to surgical decompression in a polymer-induced DCM rat model. The study assessed locomotor function, blood-spinal cord barrier (BSCB) integrity, and cell engraftment. Results showed delayed locomotor recovery upon cervical decompression and limited efficacy of BM-MSCs and BM-neurospheres in improving outcomes under the experimental conditions.
Project description:We performed single-cell RNA sequencing on neurosphere cultures derived from the dorsal telencephalic vesicles of sex segregated gestational day (GD) 12.5 C57BL/6J mouse fetuses.
Project description:Understanding the molecular bases of neurological and psychiatric conditions is hampered by a lack of suitable patient-derived cellular models. The olfactory mucosa is a continually regenerating neural tissue that contains multipotent neural stem cells which are accessible and expandable in vitro as neurospheres. Here we demonstrate disease-specific differences in neurosphere-derived cell lines from patients with two unrelated neurological conditions. Lines derived from donors with schizophrenia exhibited significant dysregulation of neurodevelopmental pathways whereas those obtained from donors with Parkinson's disease demonstrated significant dysgregulation in mitochondrial function, oxidative stress and xenobiotic metabolism. Our model supports the hypothesis that diseases with multiple genetic and environmental risk factors are revealed in convergent cellular and molecular pathways in olfactory neurosphere-derived cells taken from patient cohorts. These patient-derived cells provide informative models that provide insights into neurobiological correlates of neurological and psychiatric disorders that may be useful for many neuropsychiatric/neurodegenerative conditions and for drug discovery
Project description:We studied the membrane protein compositions of Streptococcus pneumoniae WT and scRNA mutant strains, with or without the CSP1 induction into competence state.
Project description:To determine fluoxetine-induced transcriptional signatures in GBM cancer cells, we performed RNA-sequencing analysis of three GBM patient-derived neurosphere cancer cell lines after fluoxetine or DMSO treatment. Genes with differential expression and associated transcriptional signatures were analyzed and identified in three cancer cell lines.
