Project description:In order to investigate the physiological and biochemical characteristics and molecular mechanisms during the leaf colour change of Acer rubrum L, this study used Acer rubrum L. 'Autumn Blaze' cuttings as material and analysed the transcriptome and miRNAs of Acer rubrum L leaves under different light and temperature treatments. The transcriptome and miRNAs of Acer rubrum L leaves were analysed under different light and temperature treatments, and miRNA-mRNA association analysis was performed for the differentially expressed mRNAs and miRNAs.
Project description:Norway maple and sycamore belong to the Acer genus and produce desiccation-tolerant and desiccation-sensitive seeds, respectively. We investigated the seed germination process at the imbibed and germinated stages using metabolomic and proteomic approaches to determine why sycamore seeds germinate earlier and are more successful at establishing seedlings than Norway maple seeds under controlled conditions. Embryonic axes and embryonic axes with protruded radicles were analyzed at the imbibed and germinated stages, respectively. Among the 212 identified metabolites, 44 and 67 differentially abundant metabolites were found at the imbibed and germinated stages, respectively, in both Acer species. Higher levels of amines, growth and defense stimulants, including B vitamins, were found in sycamore. We identified 611 and 447 proteins specific to the imbibed and germinated stages, respectively, in addition to groups of proteins expressed at different levels. Functional analysis of significantly regulated proteins revealed that proteins with catalytic and binding activity were enriched during germination, and proteins possibly implicated in nitrogen metabolism and metabolite interconversion enzymes were the predominant classes. Proteins associated with the control of plant growth regulation and seed defense were observed in both species at both germination stages. Sycamore proteins possibly involved in abscisic acid signal transduction pathway, stress tolerance and alleviation, ion binding and oxygenase activities appeared to accompany germination in sycamore. We identified peptides containing methionine (Met) oxidized to methionine sulfoxide (MetO), and functional analyses of proteins with significantly regulated MetO sites revealed that translation, plant growth and development, and metabolism of nitrogen compounds were the main processes under Met/MetO redox control. We propose that higher levels of storage proteins and amines together with higher levels of B vitamins supported more efficient nitrogen utilization in sycamore, resulting in faster seedling growth. In conclusion, omic signatures identified in sycamore seem to predispose germinated sycamore seeds to better postgerminative growth.
Project description:To reveal the molecular mechanism of leaf color changes in Acer pictum subsp. mono, this study was conducted on bud-transformed branches, analyzing the transcriptome and small RNAs of Acer pictum subsp. mono leaves and performing miRNA-mRNA association analysis on differentially expressed mRNAs and miRNAs.
Project description:Equine atypical myopathy (AM) is a severe environmental intoxication linked to the ingestion of protoxins contained in seeds and seedlings of the sycamore maple (Acer pseudoplatanus) in Europe. The toxic metabolites cause a frequently fatal rhabdomyolysis syndrome in grazing horses. Since toxic metabolites can also be present in co-grazing horses, it is still unclear as to why, in a similar environmental context, some horses show signs of AM whereas others remain clinically healthy. Label-free proteomics analyses on the serum of 26 diseased AM, 23 co-grazers and 11 control horses were performed to provide insights into biological processes and pathways. A total of 43 and 44 differentially expressed proteins between “AM vs co-grazing horses” and “AM vs control horses” were found. Disease-linked changes in the proteome of different groups were found to correlate with detected amounts of toxins and principal component analysis was performed to identify the 29 proteins with the greatest impact on the proteomic differences between groups. Among the pathway-specific changes the glycolysis/gluconeogenesis pathway, the coagulation/complement cascade, and the biosynthesis of amino acid were affected. Sycamore maple poisoning results in a combination of inflammation, oxidative stress, and high energy demand, which is trying to be met by enhanced glycolysis.