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Synthetic Circuits Based on Split CAS9 to Detect Cellular Events

ABSTRACT: Synthetic biology. Engineering logic circuit gates in biology to control specific functions. While CRISPR is widely used, it's not often applied to logic gates beyond controlling guide RNA. In this study, we adapted split Cas9 to create logic gates that detect biological events like cancer cell origin, epithelial to mesenchymal transition (EMT), and cell-cell fusion. We placed Cas9 halves under different promoters, making them reassemble when specific events occur. Cancer cells of epithelial origin triggered the Cas9 reporter, showing green fluorescence. Additionally, the system effectively detected EMT and combined inputs to detect cancer epithelial cells undergoing EMT. The split-Cas9 gate also sensed cell-cell fusion in induced and natural scenarios, producing multinucleated syncytia and activating the reporter. This simple split Cas9 system can be integrated into various cellular processes as a sensor and actuator.

PROVIDER: PRJEB64624 | ENA |

REPOSITORIES: ENA

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