Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

MetaPDOcheese 2: Internal transcribed spacer (ITS) region-based metabarcoding analysis of the French PDO cheeses and milks

ABSTRACT: MetaPDOcheese 2: Internal transcribed spacer (ITS) region-based metabarcoding analysis of the French PDO cheeses and milks

PROVIDER: PRJEB64628 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR12052100_1.fastq.gz	Fastqsanger.gz
	ERR12052100_2.fastq.gz	Fastqsanger.gz
	ERR12052200_1.fastq.gz	Fastqsanger.gz
	ERR12052200_2.fastq.gz	Fastqsanger.gz
	ERR12052300_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 5356

Similar Datasets

MetaPDOcheese 3: Metagenomic survey of the French PDO cheeses and milks

Project description:MetaPDOcheese 3: Metagenomic survey of the French PDO cheeses and milks

| PRJEB64630 | ENA

MetaPDOcheese 1: 16S rRNA gene-based metabarcoding analysis of the French PDO cheeses and milks

Project description:MetaPDOcheese 1: 16S rRNA gene-based metabarcoding analysis of the French PDO cheeses and milks

| PRJEB64600 | ENA

MetaPDOcheese 4: Whole genome of bacterial isolates from cheeses and milks

Project description:MetaPDOcheese 4: Whole genome of bacterial isolates from cheeses and milks

| PRJEB64629 | ENA

Project description:MetaPDOcheese 5: Genome of yeast isolates from cheeses and milks

| PRJEB91335 | ENA

Vegetable coagulants, raw milks and Caciofiore cheeses

Project description:The microbiota of Caciofiore cheeses handcrafted with different types of vegetable coagulants

| PRJNA843917 | ENA

Project description:Greek PDO Cheeses

| PRJNA687824 | ENA

Ezine PDO cheese

Project description:Ezine PDO cheese Metagenome

| PRJNA865571 | ENA

Project description:Food production is associated with significant environmental impacts. To reduce these, it is first essential to better understand the environmental implications of production processes, and how these can vary among different products. Historically, most studies in this area have focused on comparing environmental impacts among different food categories, but here, we chose to investigate differences at a finer scale, among products belonging to the same category. Cheese was selected as our study model because it is one of the most popular types of food products around the world but is associated with significant environmental impacts. Specifically, we used Life Cycle Assessment (LCA) to evaluate the environmental impacts of 44 French cheeses that benefit from Protected Designation of Origin (PDO) certification. Our assessments took into account all steps from milk production to the end of the ripening period and the functional unit used was 1 kg of fully ripened cheese. Inventories for milk production were obtained from AGRIBALYSE 3.0. Data on cheeses production stage were collected using PDO specifications, technical datasheets, scientific literature, and three expert opinions. Background inventories (electricity mix, gas production, materials, transport, water, cleaning products) were obtained from the Ecoinvent 3.6 database. Simapro software was used to conduct all LCAs, using the characterization method "EF 3.0 Method (adapted) V1.00 / EF 3.0 normalisation and weighting set". Our final dataset contains details on products, life cycle inventories (LCI), and LCIA results. These data could be useful for cheesemakers seeking to improve the environmental performance of their products.

| S-EPMC9253473 | biostudies-literature

Engineering spacer specificity of the Cre/loxP system

Project description:Translational research on the Cre/loxP recombination system focuses on enhancing its specificity by modifying Cre/DNA interactions. Despite extensive efforts, the exact mechanisms governing how Cre distinguishes between substrates remains elusive. Cre recognizes 13 bp inverted repeats, initiating recombination in the 8 bp spacer region. While literature suggests that efficient recombination proceeds between lox sites with non-loxP spacer sequences when both lox sites have matching spacers, experimental validation for this assumption is lacking. To fill this gap, we investigated target site variations of identical pairs of the loxP 8 bp spacer region, screening 6,000 unique loxP-like sequences. Approximately 84% of these sites exhibited efficient recombination, affirming the flexibility of spacer sequences for catalysis. However, certain spacers negatively impacted recombination, emphasizing sequence dependence. Directed evolution of Cre on inefficiently recombined spacers not only yielded recombinases with enhanced activity but also mutants with reprogrammed selective activity. Mutations altering spacer specificity were identified, and molecular modelling and dynamics simulations elucidated the mechanism behind this specificity switch. These findings highlight the potential to fine-tune site-specific recombinases for spacer sequence specificity, offering a novel concept to enhance the applied properties of designer-recombinases for genome engineering applications.

2024-07-25 | GSE254392 | GEO

Salmonella Dublin in Cheeses

Project description:Salmonella Dublin in Cheeses

| PRJEB28856 | ENA