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ABSTRACT:
PROVIDER: PRJEB65909 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:In this study, possible adverse effects of transgene expression in field-grown barley were assessed in relation to the influence of genetic background and to the impact of interaction with arbuscular mycorrhiza fungi. The deposited microarray data originate from a parallel transcript profiling, metabolome profiling and metabolic fingerprinting approach with the wild type cultivars Golden Promise (GP) and Baronesse (B) as well as barley transgenics with i) seed-specific expression of (1,3-1,4)-ß-glucanase (GluB) being introgressed from the Golden Promise (GP) into the cultivar Baronesse (B) and ii) ubiquitous expression of codon-optimized Trichoderma harzianum endochitinase (ChGP). Our conclusion from the results is that cultivar-specific differences and even the presence of few introgressed alleles exceed the effects on transcriptome and metabolome caused by transgene expression. Mycorrhization was shown to have more prominent effects on the metabolome than on the transcriptome.
2010-03-03 | GSE19296 | GEO
Project description:Comparison of mRNA accumulation in the seedling leaves of 8 unstressed barley cultivars ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, . The equivalent experiment is BB20 at PLEXdb.] genotype: Morex(3-replications); genotype: Steptoe(3-replications); genotype: OWB REC(3-replications); genotype: OWB DOM(3-replications); genotype: Barke(3-replications); genotype: Haruna Nijo(3-replications); genotype: Golden Promise(3-replications); genotype: Optic(3-replications)
2011-11-22 | E-GEOD-33393 | biostudies-arrayexpress
Project description:We measured mRNA abundance in the seedling leaves of the barley genotypes Golden Promise and Morex and in F1 hybrids generated using either Golden Promise as a maternal genotype and Morex as paternal or other way around. 3 biological replicates each, total 12 chips.
2007-12-31 | E-TABM-110 | biostudies-arrayexpress
Project description:Expression Data of Barley Crown and Growing Point Tissue Under Salt Stress abd JA treatment Experiment Overall Design: Barley genotype Golden Promise was used for expression anlaysis using the tissue from crown and growing point under control, salt stressed, JA treatment and JA pretreatment followed by salt stress
2008-06-13 | E-GEOD-5605 | biostudies-arrayexpress
Project description:In this study, possible adverse effects of transgene expression in field-grown barley were assessed in relation to the influence of genetic background and to the impact of interaction with arbuscular mycorrhiza fungi. The deposited microarray data originate from a parallel transcript profiling, metabolome profiling and metabolic fingerprinting approach with the wild type cultivars Golden Promise (GP) and Baronesse (B) as well as barley transgenics with i) seed-specific expression of (1,3-1,4)-ß-glucanase (GluB) being introgressed from the Golden Promise (GP) into the cultivar Baronesse (B) and ii) ubiquitous expression of codon-optimized Trichoderma harzianum endochitinase (ChGP). Our conclusion from the results is that cultivar-specific differences and even the presence of few introgressed alleles exceed the effects on transcriptome and metabolome caused by transgene expression. Mycorrhization was shown to have more prominent effects on the metabolome than on the transcriptome. Hordeum vulgare plants were cultivated in the field in 2007 at Giessen Experimental station (165m elevation over NN, 1540h sunshine and 650mm precipitation per year in average) in replicated blocks, each consisted of eight 0.8 m × 0.5 m plots in which genotypes were randomly distributed and half of the plots were pretreated with Amykor® (containing the arbuscular fungi Glomus intraradices and Glomus mosseae). Ten pools of leaf material of at least 10 leaves per pool were sampled in the middle of the light period four months after planting. Two replicates of each genotype were subjected to microarray analysis.
2010-03-02 | E-GEOD-19296 | biostudies-arrayexpress
Project description:Barley cv. Golden Promise reference assembly
| PRJEB34691 | ENA
Project description:We measured mRNA abundance in the seedling leaves of 8 barley genotypes; Morex, Steptoe, Golden Promise, Optic, Haruna Nijo, Barke, OWB-D and OWB-R. 3 biological replicates each, total 24 chips.
2007-12-31 | E-TABM-113 | biostudies-arrayexpress
Project description:Wild-type barley cv Golden Promise and two independent RNAi knockdown lines (W1-1 And W1-7) of the WHIRLY1 gene were grown under controlled environments. Leaves were harvested after 7 or 14 days and sampled along a developmental gradient of base, mid and tip. Microarray analysis was conducted from three biological replicates using a custom designed Agilent barley microarray. Analysis of transcript abundance suggests developmental delay in WHIRLY knockdown lines with significant disruption of plastid development and transcription.
2021-09-01 | E-MTAB-9882 | biostudies-arrayexpress
Project description:Salt Stress response of salt-tolerant genotype Golden Promise compared to Maythorpe Barley1 GeneChip was used to find differential expression between two barley genotypes under control and salt stress conditions at vegetative stage of growth Keywords: genotype and treatment comparison
2007-03-31 | GSE6325 | GEO
Project description:Wild type Golden Promise barley and the frost tolerant TaCBF14 and TaCBF15 transgenic barley lines were grown in plant growth chamber in the Phytotron of ELKH ATK Agricultural Institute, Martonvasar, Hungary. After three weeks in control conditions, plants were treated by short-term cold-treatment. Samples were collected under control condition and after the short-term cold-treatment too.
2021-11-10 | E-MTAB-10781 | biostudies-arrayexpress