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The histone demethylase dLSD1 is required to achieve normal wing size and to silence Transposable Elements in epithelial cells

ABSTRACT: LSD1 regulates transposable elements

PROVIDER: PRJEB67336 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR12519757.fastq.gz	Fastqsanger.gz
	ERR12521176.fastq.gz	Fastqsanger.gz
	ERR12519778.fastq.gz	Fastqsanger.gz

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Transposable elements silencing by the histone demethylase dLSD1 is required to achieve normal wing size

Project description:Determination of tissue size depends on the proper response of cells to a wide array of external and internal signals. Chromatin acts as a platform/substrate of signal integration and storage. Signal transduction pathways converge upon chromatin modifying enzymes and transcription factors to reprogram gene expression. However, little is known about the role of chromatin modifying factors in determining tissue size. Here, we show that loss of function of the histone demethylase dLSD1 results in a significant reduction of Drosophila wing size due to a decreased cell number. We show that dLSD1 depletion affects proliferation and induces DNA damage in larval wing imaginal discs. Through transcriptomics analysis, we find that dLSD1 controls the expression of multiple gene networks in wing imaginal discs. We then show that normal dLsd1 function contributes to repressing Transposable Element (TE) transcription and mobility. Additionally, our results suggest that increased TE mobility due to dLsd1 depletion contributes directly to altered organ size.

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The histone demethylase dLsd1 regulates organ size by silencing transposable elements.

Project description:The specific role of chromatin modifying factors in the timely execution of transcriptional changes in gene expression to regulate organ size remains largely unknown. Here, we report that in Drosophila melanogaster depletion of the histone demethylase dLsd1 results in the reduction of wing size. dLsd1 depletion affects cell proliferation and causes an increase in DNA damage and cell death. Mechanistically, we have identified Transposable Elements (TEs) as critical dLsd1 targets for organ size determination. We found that upon dLsd1 loss many TE families are upregulated, and new TE insertions appear. By blocking this new TE activity, we could rescue the wing size phenotype. Collectively, our results reveal that the histone demethylase dLsd1 and maintenance of TE homeostasis are required to ensure proper wing size.

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Transposable elements silencing by the histone demethylase dLSD1 is required to achieve normal wing size

Project description:Transposable elements silencing by the histone demethylase dLSD1 is required to achieve normal wing size

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