Project description:Transcriptional profiling of Haloarcula hispanica wild-type strain cultured in different media comparing MG medium with 168 medium. H. hispanica wild-type strain can accumulate PHBV in MG medium, while can not in 168 medium. Goal was to explore the PHA biosynthesis and its impact on central metabolism in H. hispanica. Total RNA from MG and 168 were used to generate target cDNA, and then hybridized to 8*15K Haloarcula hispanica genome array genechips, representing about 3800 genes.
Project description:Transcriptional profiling of Haloarcula hispanica wild-type strain cultured in different media comparing MG medium with 168 medium. H. hispanica wild-type strain can accumulate PHBV in MG medium, while can not in 168 medium. Goal was to explore the PHA biosynthesis and its impact on central metabolism in H. hispanica.
Project description:Transcriptional profiling of Haloarcula hispanica comparing PHB with 960. PHB represents the PHA synthase genes mutant (i.e., M-NM-^TphaEC) which is deficient in PHBV accumulation when grown in MG medium. 960 represents the wild-type strain which can produce PHBV when grown in MG medium. Goal was to explore the PHBV biosynthesis and its impact on central metabolism in H. hispanica. Total RNA from PHB and 960 were used to generate target cDNA, and then hybridized to 8*15K Haloarcula hispanica genome array genechips, representing about 3800 genes.
Project description:Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) from TrmB in the halophilic archaeon Haloarcula hispanica in the presence and absense of glucose.
Project description:Transcriptional profiling of Haloarcula hispanica comparing PHB with 960. PHB represents the PHA synthase genes mutant (i.e., ΔphaEC) which is deficient in PHBV accumulation when grown in MG medium. 960 represents the wild-type strain which can produce PHBV when grown in MG medium. Goal was to explore the PHBV biosynthesis and its impact on central metabolism in H. hispanica.
Project description:Transcripome profiling (RNA-seq) of TrmB in the halophilic archaeon Haloarcula hispanica in the presence and absense of glucose after 24 hours.
Project description:Salvia hispanica L. (chia) is a member of the mint family that is cultivated for its seeds. The majority of seed content in chia is comprised of omega fatty acids. Furthermore, chia seeds are also rich in fiber and minerals. The human health potential of chia seeds have driven studies of dietary effects, however there is little genetic or genomic studies available. In this study we obtained RNA from seeds, shoots, cotyledons, leaf primordia, nodes, racemes, and flower tissues from different developmental stages to generate an expression atlas for chia. RNA was sequenced on an Illumina Hiseq 2500. Sequence reads were assembled de novo to produce transcripts. Sequence reads were aligned to the chia transcriptome assembly to generate counts for each tissue type. Differentially expressed transcripts were determined for each tissue type.
