Project description:These are CUT&RUN analyses performed with embryonic day 12 (E12) CD1 mouse neocortices. Targets were ZBTB7A, GATAD2B, H3K4Me3, H3K4Me1, H3K27Ac, and IgG control.
Project description:SOX6 CUT&RUN on HUDEP1 over expressing SOX6-Flag. The experiment is done using and anti Flag Ab to assist the genome wide binding profile of SOX6 in HUDEP1 (Human Umbilical cord blood-Derived Erythroid Progenitor-1).
Project description:This study investigates the role of ADNP in regulating CHD4 and MBD3 binding using CUT&RUN. CUT&RUN was performed to capture changes in the binding of CHD4 (using FLAG-tag) and MBD3. Two biological replicates were processed for each protein with two technical replicates. This dataset aims to characterise the binding of CHD4 and MBD3 in mouse ES cells.
Project description:Here we describe successful implementation of CUT&RUN for profiling protein-DNA interactions in zebrafish embryos. We apply modified a CUT&RUN method to generate high resolution maps of enrichment for H3K4me3, H3K27me3, H3K9me3, and RNA polymerase II during zebrafish gastrulation. Using this data, we identify a conserved subset of developmental genes that are enriched in both H3K4me3 and H3K27me3 during gastrulation, and we demonstrate the increased effectiveness of CUT&RUN for detecting protein enrichment at repetitive sequences with reduced mappability. Our work demonstrates the power of combining CUT&RUN with the strengths of the zebrafish system to better understand the changing embryonic chromatin landscape and its roles in shaping development.
Project description:H3K4me3 occupancy profiles were generated for wild-type mESCs using the CUT&RUN assay. Matched non-targeting IgG samples were included as negative controls to ensure specificity of enrichment.