Project description:Transcriptome analysis was performed to systematically compare human tuberculous ulcers with non-tuberculous skin ulcers. Focusing on the former, this study aimed to identify key associated genes, elucidate their expression profiles and functional attributes, and preliminarily dissect the molecular mechanisms governing their pathological progression. By bridging the current gap in the genetic characterization of tuberculous ulcers, this research not only provides novel targets for investigating disease pathogenesis but also establishes an experimental foundation for early clinical diagnosis and targeted therapeutic strategies, thereby holding significant implications for advancing disease prevention and management.(This study used Agilent Human lncRNA V6 microarray. Only normalized expression data were submitted, original raw scan files are not available.)
Project description:We performed RNAseq on ulcers and distant normal tissue from biopsy-wounded wild-type and Bmp4-overexpressing (Vil1-Cre;Rosa26-Bmp4) mice to investigate differences in gene expression between tissue states and genotypes. Biopsy wounding was performed by taking three biopsies from well-separated areas in the colon wall using forceps. Small tissue pieces were excised from the ulcers and distant normal colon tissue and collected for RNA sequencing.