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Bulk RNA-seq of trigeminal ganglia from C57BL/6 (wild-type) and Rag1KO mice with dry eye (extraorbital lacrimal gland excision model) versus sham-operated controls

ABSTRACT: Bulk RNA-seq of trigeminal ganglia from C57BL/6 (wild-type) and Rag1KO mice with dry eye (extraorbital lacrimal gland excision model) versus sham-operated controls

PROVIDER: PRJEB74283 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR12798070_1.fastq.gz	Fastqsanger.gz
	ERR12798070_2.fastq.gz	Fastqsanger.gz
	ERR12798071_1.fastq.gz	Fastqsanger.gz
	ERR12798071_2.fastq.gz	Fastqsanger.gz
	ERR12798072_1.fastq.gz	Fastqsanger.gz

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Bulk RNA-seq of trigeminal ganglia from C57BL/6 (wild-type) and Trpv1KO mice with uni- and bilateral dry eye (uni- and bilateral extraorbital lacrimal gland excision model) versus sham-operated controls

Project description:The uni- and bilateral excision models allow comparison of the combined effects of ocular desiccation and neuroinflammation on trigeminal gene expression vs the isolated effects of neuroinflammation propagated from the other side. Also, the transient receptor potential vanilloid 1 (Trpv1)-deficient mice allow the assessment of the role of this channel. In the unilateral excision model, only the left trigeminal ganglia were analyzed, which were contralateral to the desiccated eyes (right eyes) secondary to excision of the right-side extraorbital lacrimal glands, as the left-side lacrimal glands were left untouched. Eight-week-old female mice of both strains were either operated on day 1 (either bilateral or unilateral -right-side- extraorbital lacrimal gland excision or sham surgery) and allowed to develop a dry eye state until day 10, when they were euthanized and the trigeminal ganglia were excised for RNA extraction. The two trigeminal ganglia of each mouse were pooled in one sample for the bilateral excision or sham groups whereas only the left trigeminal ganglion of each mouse was harvested for the contralateral (unilateral) group. There were 18 samples in total (2 x 3 design, 2 strains, 3 treatments) including 3 replicates/group.

2025-07-01 | E-MTAB-15193 | biostudies-arrayexpress

Bulk RNA-seq of trigeminal ganglia from C57BL/6 (wild-type) and Rag1KO mice with dry eye (extraorbital lacrimal gland excision model) versus sham-operated controls

Project description:Comparison of the effect of ocular surface desiccation and the lack of adaptive immunity (T and B cells) on gene expression in the trigeminal ganglia. Eight-week-old female mice of both strains were either operated on day 1 (either bilateral extraorbital lacrimal gland excision or sham surgery) and allowed to develop a dry eye state until day 10, when they were euthanized and the trigeminal ganglia were excised for RNA extraction. The two trigeminal ganglia of each mouse were pooled in one sample. There were 12 samples in total (2 x 2 design, 2 strains, 2 treatments).

2024-04-01 | E-MTAB-13945 | biostudies-arrayexpress

Project description:Bulk RNA-seq of trigeminal ganglia from C57BL/6 (wild-type) and Trpv1KO mice with uni- and bilateral dry eye (uni- and bilateral extraorbital lacrimal gland excision model) versus sham-operated controls

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Transcription profiling of rat lacrimal gland samples from normal, sham and operated animals

Project description:Previous studies showed that loss of muscarinic parasympathetic input to the lacrimal gland (LG) leads to a dramatic reduction in tear secretion and profound changes to LG structure. In this study, we used DNA microarrays to examine the regulation of the gene expression of the genes for secretory function and organization of the LG. Long-Evans rats anesthetized with a mixture of ketamine/xylazine (80:10 mg/kg) underwent unilateral sectioning of the greater superficial petrosal nerve, the input to the pterygopalatine ganglion. After 7 days, tear secretion was measured, the animals were killed, and structural changes in the LG were examined by light microscopy. Total RNA from control and experimental LGs (n = 5) was used for DNA microarray analysis employing the U34A GeneChip. Control rat lacrimal gland samples representing a normal unoperated, a sham, and paired contralateral controls. Experimental LG samples representing paired and unpaired contralateral controls. Paired contralateral control LGs represent LG from the same animal, one side was operated and the other side unoperated.

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Alterations in gene expression in lacrimal and salivary glands of male NOD mice due to LTBR-Ig treatment relative to control antibody

Project description:NOD mice were injected once a week with LTBR-Ig to block the LTBR-pathway, or with control monoclonal antibody MOPC from age 8 to 16 weeks old. Extraorbital lacrimal glands or submaxillary glands were dissected and total mRNA prepared. Each sample was either the combined lacrimals (2) from each mouse or individual salivary glands. There were 4 mice in each treatment group. Total mRNA was isolated and the quality was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA). Reverse transcription to prepare cDNA was performed using Invitrogen M-MLV system. The purpose was to determine changes in gene expression in glands due to blockade of the LTBR-pathway. Differential Gene Expression in NOD mouse lacrimal and salivary glands after LTBR-Ig treatment

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Analysis of the heterogeneity and complexity of murine extraorbital lacrimal gland via single-cell RNA sequencing

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Transcriptome changes in trigeminal ganglia in the mouse chronic constriction injury of the infraorbital nerve model - role of TRPM3.

Project description:In this project, we performed bulk RNAseq of mouse trigeminal ganglia at different time points following a constriction of the infraorbital nerve (IoN-CCI model). Samples include both ipsilateral and contralateral samples, as well as samples from sham-operated mice. To evaluate a possible role of TRPM3, trigeminal ganglia were isolated from both wild type and TRPM3-knockout mice. Our data indicate profound and time-dependent transcriptomic changes induced by the IoN-CCI model, but did not reveal IoN-CCI-specific differences between wild type and TRPM3-knockout mice.

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RNA-Seq of embryonic, adult lacrimal gland and lacrimal gland stem cells

Project description:The transcriptional expression analysis of 17.5, 19.5 days embryonic lacrimal gland (LG), adult lacrimal gland, lacrimal gland stem cells (LGSCs) P2 cultured for 5, 7, 10, 14 days and LGSCs P6, P10, P20 cultured for 7 days, respectively.

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Human Lacrimal Gland Gene Expression

Project description:Background: The study of human lacrimal gland biology and development is limited. Lacrimal gland tissue is damaged or poorly functional in a number of disease states including dry eye disease. Development of cell based therapies for lacrimal gland diseases requires a better understanding of the gene expression and signaling pathways in lacrimal gland. Differential gene expression analysis between lacrimal gland and other embryologically similar tissues may be helpful in furthering our understanding of lacrimal gland development. Methods: We performed global gene expression analysis of human lacrimal gland tissue using Affymetrix ® gene expression arrays. Primary data from our laboratory was compared with datasets available in the NLM GEO database for other surface ectodermal tissues including salivary gland, skin, conjunctiva and corneal epithelium. Results: The analysis revealed statistically significant difference in the gene expression of lacrimal gland tissue compared to other ectodermal tissues. The lacrimal gland specific, cell surface secretory protein encoding genes and critical signaling pathways which distinguish lacrimal gland from other ectodermal tissues are described. Conclusions: Differential gene expression in human lacrimal gland compared with other ectodermal tissue types revealed interesting patterns which may serve as the basis for future studies in directed differentiation among other areas.

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Alterations in gene expression in lacrimal and salivary glands of male NOD mice due to LTBR-Ig treatment relative to control antibody

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