Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Proof of principle for iPS2-10X-seq: cardioids experiment during 3D cardiac differentiation

ABSTRACT: Proof of principle for iPS2-10X-seq: cardioids experiment during 3D cardiac differentiation

PROVIDER: PRJEB75583 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR13071993_1.fastq.gz	Fastqsanger.gz
	ERR13071993_2.fastq.gz	Fastqsanger.gz
	ERR13071994_1.fastq.gz	Fastqsanger.gz
	ERR13071994_2.fastq.gz	Fastqsanger.gz
	ERR13071995_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

Proof of principle for iPS2-10X-seq: timecourse experiment during 2D cardiac differentiation

Project description:Proof of principle for iPS2-10X-seq: timecourse experiment during 2D cardiac differentiation

| PRJEB75579 | ENA

P. vivax: proof-of-principle global transcription analysis from two wild isolates

Project description:The complete genome sequence of the P. vivax Sal-1 strain allowed the design of a first version array representing 1 oligo/2 kb of coding sequences (http://zblab.sbs.ntu.edu.sg/vivax/index.html). Here, proof-of-principle experiments using total RNA of parasites obtained from the Sal-1 strain, from P. falciparum and from parasites obtained directly from two human patients are presented. To determine the extent of cross-hybridization of P. falciparum with P. vivax, and to determine overlaps in expression profiles of the P. vivax Sal1 monkey-adapted strain vs wild isolates, single dual hybridization analyses were performed.

2008-04-12 | E-GEOD-11075 | biostudies-arrayexpress

Pre-clinical proof of principle for orally delivered Th17 antagonist miniproteins

Project description:Pre-clinical proof of principle for orally delivered Th17 antagonist miniproteins

| PRJNA1096346 | ENA

P. vivax: proof-of-principle global transcription analysis from two wild isolates

2008-04-12 | GSE11075 | GEO

iPS2-10X-seq Neural organoid differentiation

Project description:iPS2-10X-seq Neural organoid differentiation

| PRJEB91672 | ENA

P. vivax: proof-of-principle global transcription analysis from two wild isolates

Project description:P. vivax: proof-of-principle global transcription analysis from two wild isolates

| PRJNA107065 | ENA

iPS2-multi-seq on early pasage (P3) iPS2-seq targeted hiPSC

Project description:iPS2-multi-seq on early pasage (P3) iPS2-seq targeted hiPSC

| PRJEB91715 | ENA

Size-Modulated Mesoderm-Endoderm Divergence and Myocardial Cavitation in Micropatterned Cardioids

Project description:The human heart, originating from the splanchnic mesoderm, is the first functional organ to develop, co-evolving with the foregut endoderm through reciprocal signaling. Previously, cardioid models offered new insights on cardiovascular cell lineages and tissue morphogenesis during heart development, while mesoderm-endoderm crosstalk remain incompletely understood. Here, we integrated micropatterned cardioids, CRISPR-engineered reporter hiPSCs, deep-tissue imaging, and single-cell RNA sequencing (scRNA-seq) to explore synergistic mesoderm-endoderm co-development. scRNA-seq with PHATE trajectory mapping reconstructed lineage bifurcations of mesoderm-heart and endoderm-foregut lineages, identifying key cell types in cardiac and hepatic development. Ligand-receptor interaction analysis highlighted mesodermal cells enriched in non-canonical WNT, NRG, and TGF-β signaling, while endodermal cells exhibited VEGF and Hedgehog activity. We found that micropattern sizes influenced cellular composition, cardioid cavitation, contractile functions, and mesoderm-endoderm signaling crosstalk. The cardioids generated from 600 µm diameter circle patterns showed larger cavity formation resembling early heart chamber formation. Our findings establish micropatterned cardioids as a model for mesoderm-endoderm co-development, enhancing our understanding of heart-foregut synergy during early embryogenesis.

2025-04-02 | GSE293554 | GEO

Characterisation of dysplastic liver nodules using low-pass sequencing and the proof of principle on liquid biopsies

Project description:Characterisation of dysplastic liver nodules using low-pass sequencing and the proof of principle on liquid biopsies

| PRJEB20592 | ENA

iPS2-10X-seq experiment on specific clones targeting with an shRNA either SMAD2 or B2M

Project description:iPS2-10X-seq experiment on specific clones targeting with an shRNA either SMAD2 or B2M

| PRJEB91669 | ENA