Project description:In order to study essential genomic elements in bacteria we prepare pMT85 and pMTnTetM438 mini‐transposon mutant libraries of M. pneumoniae. The dataset contains controls and the minitransposon libraries, after DNAs isolation the libraries and the controls were prepared for sequencing by HITS using standard Illumina paired‐end protocol.
Project description:Mouse primary hepatocytes (MPH) prepared from C57Bl6 male adult mice were treated with the ERS-drug thapsigargin and used to prepare RNA.
Project description:One gastrocnemius muscle was injected with the ERS-drug tunicamycin, the other with control saline solution and muscles were used to prepare RNA.
Project description:To identify the direct targets of Zeb1 we performed ChIP-seq of wild type cDC1 cell line in unstimulated condition. cDC1 cell line was used for Chromatin Immunoprecipitation, it was then fixed and crosslinked and then fragmented and the fragmented DNA-protein was immunoprecipated using Zeb1 antibody. The chromatin sample was then used to prepare library using NEB kit following the manufacturer's protocol
Project description:Livers from wild-type (WT) or NFIL3 knock-out (NFIL3 KO) C57Bl6 mal adult mice treated with the ERS-drug tunicamycin were used to prepare RNA.
Project description:Retinas from various developmental stages at postnatal p5, p11, and p27 from miRNA183/182/96 cluster knockout mice versus wild type controls were used to prepare total RNA for RNA-sequencing.
Project description:BAT obtained from embryos at E14.5, E15.5 or E16.5 of C57Bl6J mice used to prepare RNA which was then processed for analysis using MoGene-2_1-st Affymetrix microarrays according to standard procedures.
