Salmonella Typhi H58 antibiotic resistance island
Ontology highlight
ABSTRACT:
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| Other | ||||
| Other | ||||
| ERR668456_1.fastq.gz | Fastqsanger.gz | |||
| ERR668456_2.fastq.gz | Fastqsanger.gz | |||
| ERR668457_1.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 5 of 6 |
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Project description:Salmonella Typhi H58 antibiotic resistance island
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Project description:Salmonella strains encounter significant acid stress during gastrointestinal infection and within the intra-phagosome environment. Comparative studies on acid adaptation of typhoidal and non-typhoidal Salmonella (NTS) are limited. In this study, we compared the growth of S. Typhimurium 14028s and S. Typhi (Ty2) across pH range of 3-8 and observed that Salmonella enterica serovar Typhimurium exhibit enhanced growth at pH 4.5 compared to S. Typhi. Comparative transcriptomic profiling of S. Typhimurium and S. Typhi at acidic and neutral pH identified numerous differentially expressed genes (DEGs) in both the serovars. These DEGs include genes encoding membrane proteins, transcriptional regulators, and stress response proteins. Targeted deletion of these genes in S. Typhimurium significantly suppressed growth at acidic pH, confirming their role in acid response. Notably, we found that these resistance mechanisms are compromised in S. Typhi due to pseudogenization of several key genes. Heterologous expression of these pseudogenized genes in S. Typhi augmented its acid tolerance. Collectively, our findings suggest that multiple genes essential for acid survival in S. Typhimurium have become dispensable or lost in S. Typhi reflecting divergent evolutionary strategies between these two serovars.
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Project description:Azithromycin resistance Salmonella Typhi in India
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Project description:Global expression profiles of Salmonella typhi grown in the supernatant of infection and within human macrophages at 0h, 2h, 8h and 24h were obtained. Stringent analytical methods were used to compare Salmonella typhi cDNAs and revealed that known virulence factors, such as the SPI-1 and SPI-2 encoded type III secretion systems, were found to be expressed as predicted during infection by Salmonella. Intracellular Typhi expressed many genes encoding antimicrobial peptides, used the glyoxylate bypass for fatty acid utilization, and, did not induce the SOS response or the oxidative stress response. Genes coding for the flagellar apparatus, chemotaxis and the iron transport system were down-regulated in vivo. The combined use of SCOTS and microarray is an effective way to determine global bacterial gene expression profiling in the context of host infection, without the need of increasing the multiplicity of infection beyond what is seen in nature. Keywords: Time course
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Project description:Salmonella enterica subsp. enterica contains more than 2,600 serovars of which four are of major medical relevance for humans. While the typhoidal serovars (Typhi and Paratyphi A) are human-restricted and cause enteric fever, non-typhoidal Salmonella serovars (Typhimurium and Enteritidis) have a broad host range and predominantly cause gastroenteritis. In this study, we compared the core proteomes of Salmonella Typhi, Paratyphi A, Typhimurium and Enteritidis using contemporary proteomics. Five isolates, covering different geographical origins, and one reference strain per serovar were grown in vitro to the exponential phase. Protein levels of orthologous proteins between serovars were compared and subjected to gene ontology term enrichment and inferred regulatory interactions. Differential expression of the core proteomes of the typhoidal serovars appears mainly related to cell surface components and, for the non-typhoidal serovars, to pathogenicity. Our findings may guide future development of novel diagnostics and vaccines, and understanding of disease progression.
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