Project description:Gel-based liquid chromatography-tandem mass spectrometry (GeLC-MS/MS), a couple of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and LC-MS/MS has emerged as a promising tool for profiling protein expression in saliva and tissue of canine oral tumors, not yet in serum samples. This study aimed to identify serum biomarkers for canine oral tumors, specifically oral melanoma (OM) and oral squamous cell carcinoma (OSCC), compared with non-oral cancer conditions (benign tumors (BN) and controls (a healthy and periodontitis group (CTRL)) using GeLC-MS/MS.
Project description:Canine acanthomatous ameloblastoma (CAA) is a locally invasive benign oral neoplasm. Clinically, differentiating CAA from canine oral squamous cell carcinoma (COSCC) has proven to be difficult as they share many clinical, radiological, and histological features. Molecularly, even though they both have an overactive MAPK pathway in comparison to healthy gingiva tissue, their mutational landscape is distinct. Bulk RNA sequencing (RNA-seq) has uncovered pronounced differences between CAA and COSCC in programs related to, among others, hypoxia, PI3K-AKT signaling, and cell proliferation.
Project description:Tumors frequently found in dogs include canine oral tumors, either cancerous or noncancerous. The bloodstream is an important route for tumor metastasis, particularly for late-stage oral melanoma (LOM) and late-stage oral squamous cell carcinoma (LOSCC). The present study aimed to investigate serum peptidome-based biomarkers of dogs with early-stage oral melanoma, LOM, LOSCC, benign oral tumors, chronic periodontitis and healthy controls, using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and liquid chromatography tandem mass spectrometry.
Project description:Common overexpressing genes were identified in all human oral squamous cell carcinoma tissues and/or cultured cells. Ten oral squamous cell carcinoma tissues and 10 human oral squamous cell carcinoma cell lines were analyzed. Three normal oral mucosa tissues and a human non-neoplastic keratinocyte cell lines were used as control samples.
Project description:Spontaneous canine head and neck squamous cell carcinoma (HNSCC) represents an excellent model of human HNSCC but is greatly understudied. To better understand and utilize this valuable resource, we performed a pilot study that represents its first genome-wide characterization by investigating 12 canine HNSCC cases, of which 9 are oral, via high density array comparative genomic hybridization and RNA-seq. The analyses reveal that these canine cancers recapitulate many molecular features of human HNSCC. These include analogous genomic copy number abnormality landscapes and sequence mutation patterns, recurrent alteration of known HNSCC genes and pathways (e.g., cell cycle, PI3K/AKT signaling), and comparably extensive heterogeneity. Amplification or overexpression of protein kinase genes, matrix metalloproteinase genes, and epithelial–mesenchymal transition genes TWIST1 and SNAI1 are also prominent in these canine tumors. This pilot study, along with a rapidly growing body of literature on canine cancer, reemphasizes the potential value of spontaneous canine cancers in HNSCC basic and translational research.
Project description:Predictive Value of MicroRNAs in the Progression of Oral Leukoplakias Comparison of 10 samples from non-progressive leukoplakias (did not turn into oral squamous cell carcinoma), with 10 samples from progressive leukoplakias (turned into oral squamous cell carcinoma w/in 5 yrs)
Project description:The baseline immune landscape of spontaneous canine HNSCC tumors was asssessed using immunohistochemistry and nanostring gene expression profiling of 34 canine oral carcinoma tumors and two normal oral mucosal tissue samples.