Project description:Primary human foreskin fibroblasts (HFF) were infected with wild-type simplex virus 1 (HSV-1) strain 17 at a multiplicity of infection (MOI) of 10. ATAC-seq libraries were prepared starting with 50,000 cells per condition following the protocol described by Buenrostro et al., Nature Methods 2013
Project description:Identification of open chromatin regions distinguishing primary human fibroblasts from abdomen, breast, and lung during proliferation or after 40 Gy X-irradiation, or after IL1-alpha or 1L1-beta treatments.
Project description:ATAC-seq was conducted to map open chromatin regions in five individual primary human melanocyte cultures. The ATAC libraries were sequenced using paired-end sequencing on an Illumina NovaSeq platform. In total, we sequenced 15 ATAC libraries from these five independent primary melanocyte cultures (C24, C27, C56, C140, C205), with three technical replicates for each culture.
Project description:Chromatin immunoprecipitation coupled with tagmentation followed by next-generation sequencing (ChIPmentation-seq) for histone H3 lysine 4 trimethylation (H3K4me3) was performed in human primary fibroblasts. H3K4me3 is a mark of active promoters and was used to identify open, transcriptionally active chromatin in primary dermal fibroblasts isolated from healthy donors.
Project description:Bulk ATAC-seq was performed on fibroblasts from 8 different healthy mouse tissues such as bone, epididymal and inguinal fat pads, omentum, liver, lung and lymph node. This data was used to examine tissue specific chromatin landscapes in fibroblasts.
