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RNA-seq of sorted cells at day 7.5 of cardiac differentiation from human embyronic stem cells

ABSTRACT: RNA-seq of sorted cells at day 7.5 of cardiac differentiation from human embyronic stem cells

PROVIDER: PRJEB77877 | ENA |

REPOSITORIES: ENA

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ATAC-seq of sorted cells at day 7.5 of cardiac differentiation from human embryonic stem cells

Project description:ATAC-seq of sorted cells at day 7.5 of cardiac differentiation from human embryonic stem cells

| PRJEB77888 | ENA

RNA-seq of sorted cells at day 7.5 of cardiac differentiation from human embyronic stem cells

Project description:The experiment aimed to resolve cellular heterogeneity in cardiac differentiation through the purification of different cell populations by lineage markers and analysis of their transcriptomes and chromatin accessibility. The differentiation protocol was designed to promote cell diversity. The addition of SB at day 2, inhibited SMAD2/3 phosphorylation and created a high BMP signalling bias to restrict cardiac differentiation in favour of other mesodermal lineages, whereas the addition of DMH1 at day 2, inhibited SMAD1/5/8 phosphorylation to create a high Activin signaling bias to promote the co-differentiation of endoderm. Cells were sorted based on SOX17-tomato and NKX2-5-GFP knock-in reporters into their major classes: Pop1 = SB_G0_T0 Pop2 = SB_G1_T0 Pop3 = CTRL_G0_T0 Pop4 = CTRL_G1_T0 Pop5 = DM_G0_T0 Pop6 = DM_G1_T0 Pop7 = DM_G1_T1 Pop8 = DM_G0_T1

2025-02-13 | E-MTAB-14276 | biostudies-arrayexpress

ATAC-seq of sorted cells at day 7.5 of cardiac differentiation from human embryonic stem cells

2025-02-13 | E-MTAB-14277 | biostudies-arrayexpress

RNA-sequencing of Rreb1+/+ and Rreb1-/- embryonic day 7.5 mouse embryos

Project description:RNA-sequencing of Rreb1+/+ and Rreb1-/- embryonic day 7.5 mouse embryos

2021-04-21 | GSE148514 | GEO

RNA-sequencing of Rreb1+/+ and Rreb1-/- embryonic day 7.5 mouse embryos

Project description:RNA-sequencing of Rreb1+/+ and Rreb1-/- embryonic day 7.5 mouse embryos

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RNA-seq of sorted cells at day 3 of cardiac differentiation based on HAND1 level

Project description:The aim of the experiment was to identify HAND1 target genes and its impact on chromatin accessibility in relation to cardiac development. A HAND1-null hESC line was used, in which a doxycycline-inducible HAND1-T2A-BFP transgene had been integrated in approximately half of the cells for HAND1 rescue / overexpression. The hESCs were differentiated with BMP4, Activin A and CHIR. On day 2.5, doxycycline was added. On day 3, cells were dissociated and sorted by BFP level using FACS. Samples were immediately processed for RNA-seq and ATAC-seq.

2025-02-13 | E-MTAB-14278 | biostudies-arrayexpress

ATAC-seq of sorted cells at day 3 of cardiac differentiation based on HAND1 level

2025-02-13 | E-MTAB-14279 | biostudies-arrayexpress

ATAC-seq of sorted cells at day 3 of cardiac differentiation based on HAND1 level

Project description:ATAC-seq of sorted cells at day 3 of cardiac differentiation based on HAND1 level

| PRJEB77907 | ENA

RNA-seq of sorted cells at day 3 of cardiac differentiation based on HAND1 level

Project description:RNA-seq of sorted cells at day 3 of cardiac differentiation based on HAND1 level

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Effect of cardiac specific depletion of one allele of Mrps5 on gene expression in heart at postnatal day 4

Project description:To investigate the function of reduction in Mrps5 expression in the regulation of cardiomyocyte proliferation and postnatal heart development, we established the mouse with cardiac specific depletion of one allele of Mrps5 through Cre-Loxp system by crossing Mrps5 fl/fl mouse with Myh6-Cre mouse. We cross Mrps5 fl/fl mouse with Myh6-Cre mouse to obtain the Mrps5 fl/+;Myh6-Cre mouse and its control littermates Mrps5 fl/+ mouse. So that one allele of Mrps5 gene was depleted upon Myh6 gene promoter drivened Cre expression at the later stage of embyronic stage. We then performed gene expression profiling analysis using data obtained from RNA-seq of Mrps5 fl/+; Myh6-Cre and Mrps5 fl/+ mouse hearts at postnatal day 4.

2025-04-17 | GSE200939 | GEO

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