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ATAC-seq of sorted cells at day 7.5 of cardiac differentiation from human embryonic stem cells

ABSTRACT: ATAC-seq of sorted cells at day 7.5 of cardiac differentiation from human embryonic stem cells

PROVIDER: PRJEB77888 | ENA |

REPOSITORIES: ENA

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RNA-seq of sorted cells at day 7.5 of cardiac differentiation from human embyronic stem cells

Project description:RNA-seq of sorted cells at day 7.5 of cardiac differentiation from human embyronic stem cells

| PRJEB77877 | ENA

ATAC-seq of sorted cells at day 7.5 of cardiac differentiation from human embryonic stem cells

Project description:The experiment aimed to resolve cellular heterogeneity in cardiac differentiation through the purification of different cell populations by lineage markers and analysis of their transcriptomes and chromatin accessibility. The differentiation protocol was designed to promote cell diversity. The addition of SB at day 2, inhibited SMAD2/3 phosphorylation and created a high BMP signalling bias to restrict cardiac differentiation in favour of other mesodermal lineages, whereas the addition of DMH1 at day 2, inhibited SMAD1/5/8 phosphorylation to create a high Activin signaling bias to promote the co-differentiation of endoderm. Cells were sorted based on SOX17-tomato and NKX2-5-GFP knock-in reporters into their major classes: Pop1 = SB_G0_T0 Pop2 = SB_G1_T0 Pop3 = CTRL_G0_T0 Pop4 = CTRL_G1_T0 Pop5 = DM_G0_T0 Pop6 = DM_G1_T0 Pop7 = DM_G1_T1 Pop8 = DM_G0_T1

2025-02-13 | E-MTAB-14277 | biostudies-arrayexpress

RNA-sequencing of Rreb1+/+ and Rreb1-/- embryonic day 7.5 mouse embryos

Project description:RNA-sequencing of Rreb1+/+ and Rreb1-/- embryonic day 7.5 mouse embryos

2021-04-21 | GSE148514 | GEO

RNA-seq of sorted cells at day 7.5 of cardiac differentiation from human embyronic stem cells

2025-02-13 | E-MTAB-14276 | biostudies-arrayexpress

RNA-sequencing of Rreb1+/+ and Rreb1-/- embryonic day 7.5 mouse embryos

Project description:RNA-sequencing of Rreb1+/+ and Rreb1-/- embryonic day 7.5 mouse embryos

| PRJNA624861 | ENA

Gene expression profile of cells from Hey2 enhancer-LacZ transgenic (Tg) mouse embryos at embryonic day (E) 7.5.

Project description:Gene expression profile of cells from Hey2 enhancer-LacZ transgenic (Tg) mouse embryos at embryonic day (E) 7.5.

| PRJNA945411 | ENA

RNA-seq of sorted cells at day 3 of cardiac differentiation based on HAND1 level

Project description:The aim of the experiment was to identify HAND1 target genes and its impact on chromatin accessibility in relation to cardiac development. A HAND1-null hESC line was used, in which a doxycycline-inducible HAND1-T2A-BFP transgene had been integrated in approximately half of the cells for HAND1 rescue / overexpression. The hESCs were differentiated with BMP4, Activin A and CHIR. On day 2.5, doxycycline was added. On day 3, cells were dissociated and sorted by BFP level using FACS. Samples were immediately processed for RNA-seq and ATAC-seq.

2025-02-13 | E-MTAB-14278 | biostudies-arrayexpress

ATAC-seq of sorted cells at day 3 of cardiac differentiation based on HAND1 level

2025-02-13 | E-MTAB-14279 | biostudies-arrayexpress

Whole-transcriptome splicing profiling of embryonic day (E)7.5 mouse primary germ layers [RNA-seq]

Project description:Whole-transcriptome splicing profiling of embryonic day (E)7.5 mouse primary germ layers [RNA-seq]

| PRJNA438679 | ENA

Whole-transcriptome splicing profiling of embryonic day (E)7.5 mouse primary germ layers [RNA-seq]

Project description:Alternative splicing (AS) and alternative promoter (AP) usage expand the repertories of mammalian transcriptome profiles and thus diversify gene functions. However, our knowledge about the extent and functions of AS and AP usage in mouse early embryogenesis remains elusive. Here, by performing whole-transcriptome splicing profiling with high-throughput next generation sequencing, we report that AS extensively occurs in embryonic day (E) 7.5 mouse primary germ layers, and may be involved in multiple developmental processes. In addition, numerous RNA splicing factors are differentially expressed and alternatively spliced across the three germ layers, implying the potential importance of AS machinery in shaping early embryogenesis. Notably, AP usage is remarkably frequent at this stage, accounting for more than one quarter (430/1648) of the total significantly different AS events. Genes generating the 430 AP events participate in numerous biological processes, and include important regulators essential for mouse early embryogenesis, suggesting that AP usage is widely used and might be relevant to mouse germ layer specification. Our data underline the potential significance of AP usage in mouse gastrulation, providing a rich data source and opening another dimension for understanding the regulatory mechanisms of mammalian early development.

2018-03-17 | GSE111955 | GEO

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