Project description:Determining the physiological effects of parasites and characterizing genes involved in host responses to infections are essential to improving our understanding of host-parasite interactions and their ecological and evolutionary consequences. This task, however, is complicated by high diversity and complex life histories of many parasite species. The use of transcriptomics in the context of wild-caught specimens can help ameliorate this by providing both qualitative and quantitative information on gene expression patterns in response to parasites in specific host organs and tissues. Here, we evaluated the physiological impact of the widespread parasite, the pike tapeworm (Triaenophorus nodulosus), on its second intermediate host, the Eurasian perch (Perca fluviatilis).
Project description:Adult pike Esox lucius was caught in the end of summer (August, 2020) in Teletskoye Lake (Altai region, west Siberia, Russia, 51°79’10’’ N, 87°30’43’’E). Parasite (Triaenophorus crassus) was retrieved from the intestine of pike and immediately frozen in liquid nitrogen. After that, the sample was lyophilized and sent in ice (-20 °C) to the Group of mass spectrometry of Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS (Moscow, Russia) where further analysis was performed.
Project description:This study aims to investigate the DNA methylation patterns at transcription factor binding regions and their evolutionary conservation with respect to binding activity divergence. We combined newly generated bisulfite-sequencing experiments in livers of five mammals (human, macaque, mouse, rat and dog) and matched publicly available ChIP-sequencing data for five transcription factors (CEBPA, HNF4a, CTCF, ONECUT1 and FOXA1). To study the chromatin contexts of TF binding subjected to distinct evolutionary pressures, we integrated publicly available active promoter, active enhancer and primed enhancer calls determined by profiling genome wide patterns of H3K27ac, H3K4me3 and H3K4me1.
