Project description:In this study, we used transcriptomic and hormonomic approaches to examine drought-induced changes in barley roots and leaves and its rhizosphere. By studying hormonal responses, alternative splicing events in barley, and changes in the rhizosphere microbiome, we aimed to provide a comprehensive view of barley drought-adaptive mechanisms and potential plant-microbe interactions under drought stress. This approach improved our understanding of barley adaptive strategies and highlighted the importance of considering plant-microbe interactions in the context of climate change.
Project description:•Carnivorous plants from the order Caryophyllales co-opted plant phytohormones from the group of jasmonates for regulation of digestive enzyme activities. But not all genera of carnivorous plants are evenly explored and the digestive physiology of the Australian carnivorous rainbow plants of the genus Byblis (order: Lamiales) is only very poorly understood. •Here, we investigated the composition of digestive enzymes in the secreted fluid of Byblis filifolia using LC/MS, measured enzyme activities and analysed the phytohormone tissue level after experimental feeding on fruit flies and coronatine application. •Several hydrolytic digestive enzymes were identified in the secreted digestive fluid which were clearly upregulated in the presence of insect prey. But in contrast to sundew plant Drosera capensis, endogenous jasmonate did not accumulate and coronatine, a molecular mimic of jasmonates, was not able to trigger enzyme secretion. •Our results document that carnivorous plant B. filifolia is fully carnivorous with their own digestive enzyme repertoire. However in contrast to carnivorous genera from the order Caryophyllales, they are not under the control of jasmonates. This indicates that jasmonates have not been co-opted for regulation of digestive enzyme activity repeatedly during evolution of carnivorous plants.
Project description:Through 8 generations of selection, our group has developed a strain of rainbow trout that exhibits high growth rates on an economically and environmentally sustainable all plant protein, high-soy diet. The selected strain is resistant to development of soy-induced enteritis, an inflammatory intestinal pathology that occurs often in high-value carnivorous aquaculture species. To better characterize the physiological mechanism behind the superior performance of the selected strain we compared the homeostatic intestinal gene expression of the select strain to that of a commercial control line of trout. Samples were collected at early life stages known to be critical in the development of host-microbe interactions in the gut of rainbow trout. All female cohorts of both strains were reared alongside starting from eggs. Intestinal samples from 5 fish per group (2 fish strains; 2 developmental stages; 20 samples total) were used to generate mRNA selected stranded RNA-seq libraries for high throughput sequencing. Reads were quantified at the transcript level prior to evaluating differential transcript usage and differential gene expression between the two strains of trout and the developmental stages.
2020-12-15 | GSE156372 | GEO
Project description:Comparative Transcriptome Analysis Reveals Molecular Evolution in the Carnivorous Utricularia
Project description:We leverage a model nitrogen-fixing plant symbiont and integrate genomics, phenotypes, and gene expression data to associate sets of gene clusters, which are frequently gained and lost in natural populations, with plant growth. We reveal novel structural and modular genetic processes with implications for the function and inheritance of symbiosis function in plant-microbe interactions.
Project description:To explore mechanisms involved in the plant-microbe interactions, we proceeded with genome-wide transcriptome analysis of Arabidopsis roots incubated with E. coli Bl21 for 24 hours. Control plants did not receive E. coli.
Project description:Transcriptomics and molecular evolutionary rate analysis of the Bladderwort (Utricularia), a Carnivorous Plant with a Minimal Genome
