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MRNA-Seq of Danio rerio exposed to different concentrations of fungicide tebuconazole against untreated control groups

ABSTRACT: mRNA-Seq of Danio rerio exposed to different concentrations of fungicide tebuconazole against untreated control groups

PROVIDER: PRJEB81171 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	ERR13769866_1.fastq.gz	Fastqsanger.gz
	ERR13769866_2.fastq.gz	Fastqsanger.gz
	ERR13769867_1.fastq.gz	Fastqsanger.gz
	ERR13769867_2.fastq.gz	Fastqsanger.gz
	ERR13769868_1.fastq.gz	Fastqsanger.gz

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mRNA-Seq of Danio rerio exposed to different concentrations of fungicide tebuconazole against untreated control groups

Project description:In the present study transcriptome analysis was employed to investigate the early molecular responses to exposure to Tebuconazole (CAS 107534-96-3). Zebrafish embryos were exposed to Tebuconazole according to OECD guidelines (OECD test No. 236). At the end of exposure time (96 hours), simultaneous RNA and protein extraction from 10 embryos was performed using a Macherey & Nagel RNA/protein extraction kit. The obtained RNA extracts were sequenced using Illumina NovaSeq 6000 system System and the obtained sequences went through bioinformatic analysis pipeline to Identify and count the detected gene sequences followed by differential gene expression analysis. Finally, potential substance specific biomarker candidates were selected based on the differential expression patterns and the biological functions investigation of the detected DEGs.

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mRNA-Seq of Danio rerio exposed to different concentrations of the fungicide metalaxyl against untreated control groups

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mRNA-Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups

Project description:mRNA-Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups

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mRNA-Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups

Project description:mRNA-Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups

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Differential hepatic gene expression in zebrafish (Danio rerio) exposed to the fungicide chlorothalonil reveals estrogenicity

Project description:Chlorothalonil (2,4,5,6-tetrachloroisophthalonitrile) is a broad spectrum fungicide used extensively in agricultural crops . The aim of this study is to analyse the effects of Chorothalonil on the gene expression profiles in zebrafish (Danio rerio), exposed to two concentrations of the fungicide in the water. Nominal concentrations were 1) Low 0.007mg/l (environmentally relevent) and 2) High 0.035mg/ml . A commercial third generation microarray for Danio rerio (Agielnt V3, 4x44k) was used to identify patterns of gene expression in male livers during a 96h toxicological assay.

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Project description:In the present study OMICs analysis was employed to investigate the early molecular responses of zebrafish embryos to exposure to the fungicide metalaxyl. Metalaxyl, a nucleic acid metabolism inhibitor according to Fungicide Resistance Action Committee (FRAC) classification, may also induce adverse effects on non-target organisms inhabiting the environment. Early molecular responses in terms of transcriptome and proteome analysis were investigated and refined to select potentially substance specific biomarker candidates for early prediction of metalaxyl toxicity in zebrafish embryos.

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Quantitative profiling of proteome extracted from zebrafish embryos exposed to different concentrations of the fungicide difenoconazole against untreated control groups.

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mRNA-Seq of Danio rerio exposed to different concentrations of the fungicide difenoconazole against untreated control groups

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