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Effect of insect meal blend on seabass growth and gut microbiota

ABSTRACT: Effect of Hermetia illucens and Tenebrio molitor meal on growth performance and gut microbiota of seabass

PROVIDER: PRJEB81551 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR13956821_1.fastq.gz	Fastqsanger.gz
	ERR13956821_2.fastq.gz	Fastqsanger.gz
	ERR13956822_1.fastq.gz	Fastqsanger.gz
	ERR13956822_2.fastq.gz	Fastqsanger.gz
	ERR13956823_1.fastq.gz	Fastqsanger.gz

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Evaluation of the metabolic effects of insect meal from Tenebrio molitor L. in the liver of growing pigs by transcriptomics

Project description:The hypothesis was tested that insect meal (IM) as protein source influences intermediary metabolism of growing pigs. To test this, 5-week-old crossbreed pigs were randomly assigned to 3 groups of 10 pigs each with similar body weights (BW) and fed isonitrogenous diets either without (CON) or with 5 % IM (IM5) or 10 % IM (IM10) from Tenebrio molitor L. for 4 weeks and liver was analyzed using transcriptomics. Transcriptomics of the liver revealed a total of 166 transcripts differentially expressed between IM10 and CON.

2019-10-02 | GSE138244 | GEO

Evaluation of the metabolic effects of insect meal from Tenebrio molitor L. in skeletal muscle of growing pigs by transcriptomics

Project description:The hypothesis was tested that insect meal (IM) as protein source influences intermediary metabolism of growing pigs. To test this, 5-week-old crossbreed pigs were randomly assigned to 3 groups of 10 pigs each with similar body weights (BW) and fed isonitrogenous diets either without (CON) or with 5 % IM (IM5) or 10 % IM (IM10) from Tenebrio molitor L. for 4 weeks and skeletal muscle was analyzed using transcriptomics. Transcriptomics of skeletal muscle revealed a total of 198 transcripts differentially expressed between IM10 and CON.

2019-10-02 | GSE138241 | GEO

Identification of peptides and proteins in insect based feed ingredients using LCMSMS

Project description:Identification of peptides and proteins of 18 different insect meal samples derived from the species Hermetia illucens (8), Tenebrio molitor (5), Alphitobius diaperinus (3) and Acheta domesticus (2). Proteins were extracted using two different extraction and solubilization methods. Protein digests were analyzed using an ESI-MS/MS maXis Impact UHR-TOF (Bruker) coupled with an UltiMate 3000 HPLC system (Thermo Scientific) using a 1 mm I.D. x 150 mm reverse phase column (Acclaim PepMap 100 C18, Thermo). Mass spectrometry data (.baf files) were converted using compassXport (Bruker DataAnalysis 4.2) and saved as mzXML files. Proteomics data analysis was conducted using the proteoQC package (version 1.18.1) in R (version 3.4.4) running in RStudio (version 1.0.143).

2019-05-01 | MSV000083737 | MassIVE

Nutrigenomics in Animal Feeding: Digital Gene Expression Analysis in Poultry Fed Tenebrio molitor Larvae Meal

Project description:The purpose of this study was to investigate the effects of high levels of Tenebrio molitor dietary inclusion (15%) on molecular mechanisms that influence poultry health in a broiler chicken diet.

2022-02-28 | GSE197444 | GEO

Proteomic insights into novel food insects: Homology-based proteome characterization and allergenicity considerations for EU-regulated insect species

Project description:Insects have emerged as a sustainable alternative protein source and recently gained regulatory approval in the European Union as novel foods and animal feed ingredients. However, the limited availability of species-specific proteomic databases presents a significant challenge for the accurate identification of proteins in insect-based food products, relevant for food authenticity, safety and allergenicity concerns. In this study, we developed a homology-based proteomic workflow to address the lack of comprehensive sequence data for edible insect species and to explore their allergenic potential. This nanoflow tandem mass spectrometry method (nano-HPLC HR-MS) aims to be generally applicable to underexplored species. We identified between 1079 and 1893 proteins for each of the six investigated insect species Acheta domesticus, Locusta migratoria, Tenebrio molitor, Alphitobius diaperinus, Gryllodes sigillatus and Hermetia illucens, increasing their numbers of known proteins up to 24-fold. Furthermore, we assessed the influence of error-tolerant peptide-spectra matching searches and database composition on protein identification and confirmed on average 90% of selected peptides using targeted methods. We consistently detected known and putative allergens across all tested species, providing further evidence on their allergenic potential. Data from this study may provide the general basis for the development of targeted parallel reaction monitoring mass spectrometry-based assays for so far unsequenced species authentication and quantification of their proteins e.g. in novel foods.

2025-06-03 | PXD061237 | Pride

Effect of replacing soybean meal with Hermetia illucens meal on cecal microbiome, liver transcriptome and plasma metabolome of broilers

Project description:Despite the existence of a number of studies investigating the effect of insect meal on the growth performance of broilers, knowledge about the metabolic effects of insect meal in broilers is still scarce. Thus, the present study investigated the effect of partial replacement of soybean meal with Hermetia illucens (HI) larvae meal on the liver transcriptome, the plasma metabolome and the cecal microbiome in broilers. For the study, 72 male one-day-old Cobb 500 broilers were divided into three groups (n = 12) and fed three different diets with either 0% (HI0), 7.5% (HI7.5) or 15% (HI15) defatted HI meal for 35 d. While body weight (BW) gain, feed intake, and feed:gain ratio did not differ between groups, breast muscle weight, carcass yield and apparent ileal digestibility (AID) of 12 amino acids were higher in group HI15 than in group HI0 (P > 0.05). Indicators of α-diversity (Chao1 and Observed) in the cecal digesta were higher in groups HI15 and HI7.5 than in group HI0 (P < 0.05). The abundance of 5 families and 18 genera, all of which belonged to the Firmicutes phylum, in the cecal digesta differed among groups (P < 0.05). Concentrations of butyric acid, valeric acid and isobutyric acid in the cecal digesta were lower in group HI15 than in the other two groups (P > 0.05), whereas those of total and other short-chain fatty acids were not different between groups. Liver transcriptomics revealed a total of 70 and 61 differentially expressed transcripts between groups HI15 vs. HI0 and between groups HI7.5 vs HI0, respectively, (P > 0.05). Targeted metabolomics identified 138 metabolites, most of which were triglyceride species, being different between the three groups (FDR < 0.05). According to this study, dietary inclusion of HI larvae meal has no detrimental impact but increases breast muscle weight and carcass weight in broilers suggesting that HI larvae meal can be recommended as a sustainable alternative protein source for broilers.

2024-02-21 | GSE255945 | GEO

Project description:Broiler Hermetia illucens larvae meal

| PRJNA1079797 | ENA

On the compatibility of fish meal replacements in aquafeeds for rainbow trout. A combined metabolomic, proteomic and histological study.

Project description:The sustainable development of modern aquaculture must rely on a significant reduction of the fish meal (FM) used in aquafeed formulations. However, FM substitution with alternative ingredients in diets for carnivorous fish species often showed reduced nutrient absorption, significantly perturbed metabolisms and histological changes at both hepatic and intestinal level. In the present study, adult rainbow trout (Oncorhynchus mykiss) were fed three different experimental aquafeed formulations. A control diet with higher FM content (27.3%) than two test formulations in which fish meal was substituted with two more sustainable and promising alternatives: insect meal (Hermetia illucens larvae=10.1%, FM=11.6%) and poultry by-products meal (PBM=14.8%; FM=11.7%). Combined metabolomics and proteomics analyses of fish liver, together with histological examination of liver and intestine demonstrated that a well balanced formulation of nutrients in the three diets allowed high metabolic compatibility of either substitutions, paving the way for innovative and sustainable use of novel raw materials for the fish feed industry. Results show that the main metabolic pathways of nutrient absorption and catabolism were essentially unaltered by alternative feed ingredients, and also histological alterations were negligible. It is demonstrated that substitution of fish meal with sustainable alternatives does not impact on fish metabolism, given proper efforts are put in fulfilling nutritional requirements of rainbow trout.

2022-07-18 | PXD033201 | Pride

Effect of poultry byproduct meal on European seabass gut microbiota

Project description:Effect of fishmeal replacement with poultry byproduct meal (PBM) on European seabass intestinal microbiota.

| PRJEB70800 | ENA

Effect of replacing soybean meal with Hermetia illucens meal on cecal microbiome, liver transcriptome and plasma metabolome of broilers

Project description:Effect of replacing soybean meal with Hermetia illucens meal on cecal microbiome, liver transcriptome and plasma metabolome of broilers

| PRJNA1077290 | ENA

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