Project description:Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and Larp7 Knock-out Staged Male Germ Cells Transcriptomes
Project description:We seprated the primodial germ cells using MACS from E12.5 stages in WT and Rif1-knock out mice, and using modified Smart-seq2 methods to get 1,000 cells library.
Project description:Analysis the difference in the transcriptomes (expression and alternative splicing) of either spermatocytes or round spermatids between Larp7 conditional knock-out and control mice