Biofilms in water distribution systems
Ontology highlight
ABSTRACT:
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| ERR732643.fastq.gz | Fastqsanger.gz | |||
| ERR732644.fastq.gz | Fastqsanger.gz | |||
| ERR732645.fastq.gz | Fastqsanger.gz | |||
| ERR732646.fastq.gz | Fastqsanger.gz | |||
| ERR732647.fastq.gz | Fastqsanger.gz |
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Project description:Biofilms on surfaces pose quality and health risks, resisting traditional antimicrobial methods. Plasma-activated water (PAW) shows promise in biofilm elimination, yet its genetic impact remains unclear. We investigated Escherichia coli biofilm responses to PAW treatment, revealing significant gene expression changes and pathway activations. Knockout mutants of key genes confirmed their role in PAW response. This study enhances our understanding of PAW's anti-biofilm mechanisms and bacterial stress responses.
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Project description:In drinking water distribution systems (DWDS), a disinfectant residual is usually applied to limit bacterial regrowth. However, delivering water with no or reduced chlorine residual could potentially decrease the selection for antimicrobial resistant microorganisms, favor bacterial regrowth and result in changes in bacterial populations. To evaluate the feasibility of water reduction in local DWDS while ensuring water safety, water quality was measured over 2 months in two different networks, each of them harboring sub-areas with normal and reduced chlorine. Water quality remained good in chlorine reduced samples, with limited development of total flora and absence of coliforms. Furthermore, 16S rRNA amplicon-based metagenomics was used to investigate the diversity and the composition of microbial communities in the sub-networks. Taxonomic classification of sequence reads showed a reduced bacterial diversity in sampling points with higher chlorine residuals. Chlorine disinfection created more homogeneous bacterial population, dominated by Pseudomonas, a genus that contains some major opportunistic pathogens such as P. aeruginosa. In the absence of chlorine, a larger and unknown biodiversity was unveiled, also highlighted by a decreased rate of taxonomic classification to the genus and species level. Overall, this experiment in a functional DWDS will facilitate the move toward potable water delivery systems without residual disinfectants and will improve water taste for consumers.
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Project description:We developed a spatially resolved method to profile the spatial transcriptome of biofilm. In detail, we used fluorescent dyes to label the different regions of biofilm cultured in a microfluidic chip. After staining, the bacterial cells in biofilm were sorted into relevant bins according to their spatial information marked by the fluorescent pattern. Finally, miniBac-seq (RNA-seq) method was applied to capture the transcriptome of each bin.
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Project description:The objective of the current study was to understand the glutaraldehyde resistance mechanisms in P. fluorescens and P. aeruginosa biofilms. Glutaraldehyde is a common biocide used in various industries to control the microbial growth. Recent reports of emergence of glutaraldehyde resistance in several bacterial species motivated this study to understand the genetic factors responsible got glutaraldehyde resistance. Using a combination of phenotypic assays, chemical genetic assays and RNA-seq, we demonstrate that novel efflux pump, polyamine biosynthesis, lipid biosynthesis and phosphonate degradation play significant role in glutaraldehyde resistance and post-glutaraldehyde recovery of Psudomonad biofilms. Examination of P. fluorescens 72 h biofilm transcriptome was elucidated upon exposure to glutaraldehyde. The results were confirmed using qRT--PCR and chemical genetic appraoches in P. fluorescens and P. aeruginosa.
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Project description:Nontuberculous mycobacteria (NTM) are ubiquitous and have been isolated from a variety of environmental sources, including water. Various NTM were isolated from biofilms in drinking water distribution systems in two urban and two semiurban areas in South Africa. Most of the isolates belonged to opportunistic pathogenic species of the NTM group, but none belonged to the Mycobacterium avium complex.
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Project description:The recovery of other pathogens in COVID-19 patients has been reported. The presence of either co-infection or superinfection with bacterial pathogens was associated with poor outcomes, including increased mortality. The recognition of possibility of SARS-CoV-2 co-infection is important as it enables the implementation of appropriate infection control measures and therapy. This is a proof-of-concept study uses in vitro approaches (including crystal violet assay, microrheology, and LC-MS-based prote-omics) to investigate the interaction between SARS-CoV-2 and biofilms of bacteria (S. pneumoniae and S. aureus). SARS-CoV-2 spike protein S1 subunit was found to suppress biofilm formation of both bacteria. The effect of coronavirus and spike protein on bac-terial biofilm was supported by proteomics data that shows variations in proteins in-volved in quorum sensing and biofilm formation/maturation. Preliminary in vitro data suggest that dispersion of opportunistic pathogens from biofilm may be associated with poor prognosis in co-infections. However, further investigations are needed to establish bacterial biofilm as a risk factor for secondary pneumonia in COVID-19 patients.
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Project description:Biofilms are ubiquitous in the pipes of drinking water distribution systems (DWDSs), and recent experimental studies revealed that the chlorination of the microbial carbon associated with the biofilm contributes to the total disinfection by-products (DBPs) formation with distinct mechanisms from those formed from precursors derived from natural organic matter (NOM). A multiple species reactive-transport model was developed to explain the role of biofilms in DBPs formation by accounting for the simultaneous transport and interactions of disinfectants, organic compounds, and biomass. Using parameter values from experimental studies in the literature, the model equations were solved to predict chlorine decay and microbial regrowth dynamics in an actual DWDS, and trihalomethanes (THMs) formation in a pilot-scale distribution system simulator. The model's capability of reproducing the measured concentrations of free chlorine, suspended biomass, and THMs under different hydrodynamic and temperature conditions was demonstrated. The contribution of bacteria-derived precursors to the total THMs production was found to have a significant dependence on the system's hydraulics, seasonal variables, and the quality of the treated drinking water. Under system conditions that promoted fast bacterial re-growth, the transformation of non-microbial into microbial carbon DBP precursors by the biofilms showed a noticeable effect on the kinetics of THMs formation, especially when a high initial chlorine dose was applied. These conditions included elevated water temperature and high concentrations of nutrients in the influent water. The fraction of THMs formed from microbial sources was found to reach a peak of 12% of the total produced THMs under the investigated scenarios. The results demonstrated the importance of integrating bacterial regrowth dynamics in predictive DBPs formation models.
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