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Transcriptomic response in planktonic and biofilm cells of S. mutans treated with sublethal concentrations of chlorhexidine

ABSTRACT: Analysis of the Streptococcus mutans UA159 transcriptome upon treatment with chlorhexidine and amoxicillin

PROVIDER: PRJEB83273 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR14150162_1.fastq.gz	Fastqsanger.gz
	ERR14150162_2.fastq.gz	Fastqsanger.gz
	ERR14150163_1.fastq.gz	Fastqsanger.gz
	ERR14150163_2.fastq.gz	Fastqsanger.gz
	ERR14150164_1.fastq.gz	Fastqsanger.gz

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Transcriptomic stress response in Streptococcus mutans following treatment with a sublethal concentration of chlorhexidine digluconate

Project description:Despite the widespread use of antiseptics such as chlorhexidine digluconate (CHX) in dental practice and oral care, the risks of potential resistance toward antiseptics in oral bacteria have only been highlighted very recently. Since the molecular mechanisms behind antiseptic resistance or adaptation are not entirely clear yet and the bacterial stress response has not been investigated systematically so far, the aim of the present study was to investigate the transcriptomic stress response in Streptococcus mutans after treatment with CHX using RNA sequencing. Planktonic cultures of stationary phase S. mutans were treated with a sublethal dose of CHX (125 µg/mL) for 5 min. After treatment, RNA was extracted, and RNA sequencing was performed on the Illumina NextSeq 500. Differential expressed genes were analyzed and validated by qRT-PCR. The analysis of the differential gene expression following pathway analysis revealed a considerable number of genes and pathways significantly regulated in S. mutans after sublethal treatment with CHX. In summary, expression of 423 genes was up-regulated and 295 genes down-regulated after CHX treatment. Analysis of differentially expressed genes and significantly regulated pathways showed regulation of genes involved in purine nucleotide synthesis, biofilm formation, transport systems and stress responses. In conclusion, these results show an overview of the transcriptomic stress response in S. mutans upon exposure to CHX and give an insight in potential mechanisms that may result in development of resistances.

2022-03-30 | GSE197633 | GEO

Streptococcus mutans UA159 control vs. Streptococcus mutans UA159 supplemented with 20 uM 4,5-dihydroxy-2,3-pentadione (DPD) at 3 different pH values

Project description:Transcriptional profiling of early logarithmic phase culture (O.D=0.2-0.3) of Streptococcus mutans UA159 comparing control of untreated Streptococcus mutans UA159 bacteria with Streptococcus mutans UA159 bacteria spplemented with 20µM synthetic DPD (pre-AI-2) which regulates gene expression via AI-2 quorum sensing system.Three compairisons were performed at pHs of 7,6 and 5.

2010-07-29 | GSE23159 | GEO

Gene expression profiling of Streptococcus mutans in biofilm and planktonic conditions

Project description:In vitro comparative transcriptome analysis was carried to determine genes that are differentially expressed in S. mutans biofilm compared with free-living cells. DNA-microarray analyses indicated that about 12 % of genes showed significant differential expression: 139 were activated and 104 were repressed in biofilm vs. planktonic environment. Keywords: cell type comparison

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The CdaA Regulon in Streptoccus mutans

Project description:Nucleotide signaling pathways are found in all kingdoms of life and are utilized to coordinate a rapid response to changes in the environment. One more recently discovered signaling nucleotide is the secondary messenger cyclic diadenosine monophosphate (c-di-AMP), which is widely distributed among bacteria and is also found in several archaea. This cyclic nucleotide has been shown to involve in several important cellular processes, including maintenance of DNA integrity, cell wall metabolism, stress tolerance, transcription regulation and virulence. However, the mechanisms by which c-di-AMP modulates these physiological changes have remained largely unknown.In the present study, we identified and characterized a c-di-AMP synthase (CdaA) in S. mutans UA159. Furthermore, we investigated the role of CdaA in S. mutans cell physiology and global gene expression by utilizing cdaA gene in-frame deletion mutant. Our findings suggest that CdaA is an important global modulator of optimal growth and environmental adaption in this pathogen. Streptococcus mutans UA159 whole-genome arrays (8 x 15 K) were obtained from Agilent and included 1998 probes for S. mutans transcripts. For microarray analysis, S. mutans UA159 and S. mutans ?cdaA cells were routinely grown at 37°C anaerobically (90% N2, 5% CO2, 5% H2) in brain heart infusion broth (BHI; Difco, Sparks, MD, USA) to an optical density at 600 nm (OD600) of 0.5. Four RNA samples isolated from four independent cultures of UA159 and cdaA mutant strains were hybridized to the arrays and analyzed.

2015-04-06 | E-GEOD-65511 | biostudies-arrayexpress

Streptococcus mutans UA159 grown in the presence of glucose or maltose pH7 and pH5

Project description:Transcriptional analysis of the effects of sugar source in S. mutans RNA was extracted from 4 replicate samples of S. mutans UA159, UA159 grown in glucose or maltose in continuous culture to steady-state pH values of 7 or 5. RNA was labeled with Cy3. For each replicate, labeled RNA was hybridized to slides along with Cy5-labeled reference RNA, extracted from S. mutans UA159 cultures grown to mid-log.

2014-06-01 | E-GEOD-55952 | biostudies-arrayexpress

Identification of the phosphotransacetylase (pta) and acetate kinase (ackA) regulons in Streptococcus mutans UA159

Project description:Transcriptional comparisons between wild-type Streptococcus mutans UA159 and two mutant strains (∆ackA and ∆ackA pta).

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Streptococcus mutans UA159 with or without 8.4% oxygen vs. nox mutant strain. pH7

Project description:Transcriptional analysis of the effects of oxygen concentraion in S. mutans RNA was extracted from 3 or 4 replicate samples of S. mutans UA159, UA159 grown in 8.4 % oxygen concentration and MU1020 (M-bM-^HM-^Fnox) grown in continuous culture to steady-state pH values of 7. RNA was labeled with Cy3. For each replicate, labeled RNA was hybridized to slides along with Cy5-labeled reference RNA, extracted from S. mutans UA159 cultures grown to mid-log.

2012-10-04 | E-GEOD-38803 | biostudies-arrayexpress

Streptococcus mutans UA159 with or without 8.4% oxygen vs. nox mutant strain. pH5

Project description:Transcriptional analysis of the effects of oxygen concentraion in S. mutans RNA was extracted from 3 or 4 replicate samples of S. mutans UA159, UA159 grown in 8.4 % oxygen concentration and MU1020 (M-bM-^HM-^Fnox) grown in continuous culture to steady-state pH values of 5. RNA was labeled with Cy3. For each replicate, labeled RNA was hybridized to slides along with Cy5-labeled reference RNA, extracted from S. mutans UA159 cultures grown to mid-log.

2014-04-01 | E-GEOD-56118 | biostudies-arrayexpress

Streptococcus mutans UA159 vs. spx mutant strains

Project description:Transcriptional profiling to investigate the regulatory roles of SpxA and SpxB of S. mutans. RNA was extracted from four replicate samples of each strain of interest (spxA mutant, spxB mutant, spxAB double-mutant, UA159 wild type) and labeled with Cy3. For each replicate, labeled RNA was hybridized to slides along with Cy5-labeled reference RNA, extracted from S. mutans UA159 cells grown to mid-log.

2010-05-16 | E-GEOD-19656 | biostudies-arrayexpress

Streptococcus mutans UA159 vs. fabT mutant strain, pH5 vs. pH7

Project description:Transcriptional analysis of the regulator FabT in S. mutans RNA was extracted from 4 replicate samples of S. mutans UA159 and MU1591 (M-bM-^HM-^FfabT) grown in continuous culture to steady-state pH values of 7 and 5. RNA was labeled with Cy3. For each replicate, labeled RNA was hybridized to slides along with Cy5-labeled reference RNA, extracted from S. mutans UA159 cultures grown to mid-log.

2012-05-31 | E-GEOD-36960 | biostudies-arrayexpress

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