Whole transcriptome sequencing of Arabidopsis thaliana flower buds under Salicylic acid and Methyl Jasmonate induction
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PROVIDER: PRJEB83417 | ENA |
REPOSITORIES: ENA
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Project description:Using microarray technology and a set of chickpea (Cicer arietinum L.) unigenes and grasspea (Lathyrus sativus L.) ESTs, chickpea responses to treatments with the defence signalling compounds salicylic acid (SA), methyl jasmonate (MeJA), and aminocyclopropane carboxylic acid (ACC) were studied in four chickpea genotypes with ranging levels of resistance to ascochyta blight (Ascochyta rabiei (Pass.) L.). The experimental system minimized environmental effects and was conducted in reference design, where samples from untreated controls acted as references against post-treatment samples. Robust data quality was achieved through the use of three biological replicates (including a dye-swap), the inclusion of negative controls, and strict selection criteria for differentially expressed genes including a fold change cut-off determined by self-to-self hybridizations, Students t test and multiple testing correction (P<0.05). Microarray observations were also validated by quantitative RT-PCR. The time-course expression patterns of 715 experimental microarray features resulted in differential expression of 425 genes in at least one condition. The A. rabiei resistant chickpea genotypes showed a more substantial range of defence-related gene induction by all treatments, indicating that they may possess stronger abilities to resist infection. Further, the involvement of SA, MeJA, and ACC signalling was identified for the regulation of some important A. rabiei responsive genes, as well as cross-talk between these pathways. This study also found evidence to suggest the involvement of A. rabiei-specific signalling mechanisms for the induction of several genes that were previously implicated in A. rabiei resistance. Overall, this study characterised the regulatory mechanisms of many chickpea genes that may be important in defence against various pathogens, as well as other cellular functions. Although the size of the microarray was limited, the results provided novel insights to the molecular control of chickpea cellular processes, which may assist the understanding of chickpea defence mechanisms and allow enhanced development of disease resistant cultivars. Keywords: time course defence-signalling teatment analysis
2007-03-01 | GSE5512 | GEO
Project description:Jasmonic acid (JA) and methyl jasmonate (MeJA) regulate plant development, resistance to stress, and insect attack by inducing specific gene expression. However, little is known about the mechanism of plant defense against herbivore attack at a protein level. Using a high-resolution 2-DE gel, we identified 60 MeJA-responsive proteins and measured protein expression level changes. Among these 62 proteins, 43 proteins levels were increased while 11 proteins were decreased. We also found eight proteins uniquely expressed in response to MeJA treatment. The proteins identified in this study have important biological functions including photosynthesis and energy related proteins (38.4%), protein folding, degradation and regulated proteins (15.0%), stress and defense regulated proteins (11.7%), and redox-responsive proteins (8.3%). We found MeJA could not only induce plant defense mechanisms to insects, it also enhanced toxic protein production that potentially can be used for bio-control of Asian corn borer.
2015-03-25 | PXD001793 | Pride
Project description:Stresses from either biotic or abiotic origins can have significant impact towards plant physiology and molecular regulation. Jasmonate acid (JA) and its derivative, methyl JA (MeJA) are hormonal cues released by plants which signal defensive response to curb the damage from such stresses. In an attempt to study the defensive response, a tropical herbal plant, Persicaria minor (P. minor) which is known for its pungent smell as well as various bioactivities including antimicrobial and anti-cancer, has been treated with MeJA to invoke the stress signaling. Such elicitation has been performed in various plants such as Arabidopsis, rice and hairy root cultures of certain herbs, yet how MeJA directly influenced the proteome of a herbal species particularly P. minor has not been previously elucidated. In this study, P. minor plants was exogenously treated with MeJA and its proteome was investigated using a new proteomics approach called SWATH-MS.
2018-06-25 | PXD005749 | Pride
Project description:MEcPP overaccumulation in the ceh1 mutant results in significant transcriptome changes as reported by microarrays, of which some are dependent on the SA accumulation in this mutant. In order to gain better resolution of transcriptomic changes in ceh1 as well as the SA dependence of these changes, we performed RNAseq analysis of RNA extracted from wt, ceh1, eds16 deficient in SA production, and ceh1/eds16
2017-06-01 | GSE96666 | GEO
Project description:Calli generated from grape berries were used to establish a cell suspension in Gamborgs B5 liquid medium with minimal organics supplemented with 30 gL-1 sucrose, 0.25 gL-1 casein hydrolysate, 0.93 ?M kinetin and 0.54 ?M naphthaleneacetic acid (NAA) and incubated in darkness at 26°C. <br>The cells were cultured in 250 mL flasks (50 mL of cell suspension in each) incubated in darkness on an orbital shaker at 100 rpm. The cell suspensions were sub-cultured every 2 weeks using an initial packed cell volume (PCV) of 15%. For the evaluation of the effect of elicitor addition on the changes in gene expression, 100 mL flasks containing 20 mL of the above cell suspension were used. Each treatment was conducted in triplicate.<br><br>In a set of preliminary experiments studying the effect of elicitors on the volatiles produced by the cell suspensions, the compounds were added during the exponential growth phase when the PCV was approximately 50%. After 72h incubation in darkness after elicitor addiction, cells were harvested. The elicitors screened for their ability to induce volatile compound production were: 500 µM methyl jasmonate (MeJA); 500 uM MeJA + 500 µM salicylic acid (SA); 500 µM jasmonic acid (JA). Controls were conducted by adding the same volume of the solvent used to dissolve each elicitor to the cultures. For the jasmonate and SA experiments the solvent was ethanol.<br>
2009-04-28 | E-MEXP-1524 | biostudies-arrayexpress
Project description:Transcriptional profiling of MeJA-treated pearl millet seedlings over time [0, 12, 24 and 48 hours post treatment (hpt)]. Keywords: Time course, Stress response
2009-01-01 | GSE13478 | GEO
Project description:Transcriptional profiling of barley shoot and root with MeJA
2014-04-01 | GSE41515 | GEO
Project description:Licorice (Glycyrrhiza uralensis Fisch) flavonoids have many pharmacological effects, as the main chemical component of licorice, its content directly affects the quality of licorice. Methyl jasmine (MeJA) is an important signaling molecule in the secondary metabolic pathway of plants, but the biological mechanisms that stimulating the production of licorice flavonoids and the related changes in transcriptome are still less studied. In this research, the expression of two key enzyme genes: Chalcone synthase (CHS) and Cinnamate 4-hydroxylase (C4H) in the biosynthesis pathway of licorice flavonoids was determined, and it was significantly different after 9 hours of MeJA induction. The transcriptome profiles of licorice cells at 9 hours after MeJA treatment were analyzed to investigate the transcriptional alterations of licorice cell in response to MeJA elicitation by “RNA-seq”. 151, 529 transcripts (200 bp in length) of cDNA from the samples were generated, and 116, 907 unigenes were found. MeJA appeared to stimulate a large number of genes involved in several relevant functional categories, such as carbohydrate metabolism and encoding transcription factors, 11 MYB transcription factors expressed significant differences were screened. This comprehensive description of gene expression information could help elucidate the molecular mechanism of MeJA-mediated biosynthesis of licorice flavonoids and MeJA-regulated network formation.
2019-08-01 | GSE128503 | GEO
Project description:The aim was to determine the changes in cell wall composition and transcriptome changes following treatment with the stress hormone precursor methyl jasmonate (MeJA) in the model grass Brachypodium distachyon. The correlation between transcript changes and cell wall composition changes allowed identification of candidate genes responsible for grass-specific features of the cell wall that are specifically changed in response to MeJA.
2017-09-04 | E-MTAB-5413 | biostudies-arrayexpress
Project description:Effect of MeJA treatment on global gene expression involved in diverse developmental and defensive processes in Arbidopsis thaliana
2006-08-24 | E-ATMX-2 | biostudies-arrayexpress