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SC5314 derived uniconazole adaptors

ABSTRACT: SC5314 derived uniconazole adaptors

PROVIDER: PRJEB86013 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	ERR14647400_1.fastq.gz	Fastqsanger.gz
	ERR14647400_2.fastq.gz	Fastqsanger.gz
	ERR14647401_1.fastq.gz	Fastqsanger.gz
	ERR14647401_2.fastq.gz	Fastqsanger.gz
	ERR14647402_1.fastq.gz	Fastqsanger.gz

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SC5314 derived uniconazole adaptors

Project description:Candida albicans lab strain SC5314 was spread on YPD+uniconazole (0.06-4μg/mL) plates. Randomly 4 adaptors from each drug plate were sequenced.

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H99 derived uniconzole (0.25ug/mL and 2ug/mL) adaptors

Project description:H99 derived 2 ug/mL uniconazole adaptors: Approximately 1 million cells of Crypotoccus neoformans lab strain H99 were spread on YPD-agar plate supplemented with 2 ug/mL uniconazole. After 5 days incubation of the plate at 30℃, randomly 30 adaptors were chosen. 21 adaptors were resistant and were sequenced. H99 derived 0.25 ug/mL uniconazole adaptors: Cells of H99 were adjusted to 2500 cells/mL. The culture was supplemented with 0.25 ug/mL uniconazole. After 72 h incubation, the culture was washed, diluted and approximately 200 cells were spread on YPD-agar plate. Randomly 95 colonies were tested for resistance to uniconazole. One colony (TJ3832) was resistant and was sequenced.

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Project description:Gene AFR1 was deleted from Cryptococcus neoformans lab strain H99. Approximately 1 million cells of the deletion strain were spread on YPD-agar plate supplemented with 0.125ug/mL uniconazole. Randomly 30 adaptors were chosen. 16 adaptors were resistant to uniconazole and were sequenced.

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Project description:YJB-T490 was spread on YPD-agar plates supplemented with miconazole (0.008-2ug/mL). Randomly 3 adaptors from each drug plate were sequenced. In addition, 2 miconazole adaptors derived from SC5314, and 6 adaptors derived from CDR1 deletion strain were also sequenced.

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Project description:Candida albicans strains SC5314 and YJB-T1891 were used as wild type to delete VMA11. The deletion strains (TJ926 and TJ927) were spread on YPD plate supplemented with 1μg/ml fluconazole. Randomly 96 adaptors derived from TJ926 and 96 adaptors derived from TJ927 were tested with disk diffusion assay. Among the resistant adaptors, randomly 15 adaptors (A4-A18) and 48 adaptors (A19-A66) derived from TJ926 were sequenced.

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Project description:Candida albicans lab strain SC53114 was used to obtain fluconazole-tolerant adaptors. 53 adaptors as well as the parent strain were sequenced.

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