Project description:Domestication of pig results in modifications of many traits, including fatness traits, which are important in pig production since they have effect on meat quality, fattening efficiency, reproduction and immunity.In this study, we investigate 3D genome organization and transcriptomic characterization of adipose tissues (ATs) between wild boars and Bama pig, a typical indigenous domestic pig in China, to uncover molecular mechanisms of fatness-phenotypic shifts.
Project description:8 animals of high feed efficiency and 8 animals of low feed efficiency, selected by the measure of residual intake and body weight gain at the end of a feeding trial, had their liver samples collected by biopsy and sequenced in a Illumina HiSeq2500 plataform
Project description:Porcine embryonic germ cells (EGC) are cultured pluripotent cells derived from primordial germ cells (PGC). This study explored the possibilities to establish porcine EGC lines in the domestic breed pig more efficiently and from earlier embryonic stages than reported to date. In vitro culture of PGC from both pooled and individual embryos at days 17-24 of gestation resulted in the successful derivation of putative EGC lines from days 20-24 with high efficiency, while no lines could be established from days 17-18. The EGC-like colonies had characteristic morphology and electron microscopy revealed tight junctions and presence of primary cilia on the cell surfaces. The cells formed simple embryoid bodies in suspension culture and further differentiated into epithelial-like, mesenchymal-like, and neuronal-like cells. Our results show that putative porcine EGC can be derived from migrating PGC with high efficiency using individual embryos from different genetic backgrounds. RNA-Seq profiling of 2 different in vitro cultures of pig embryonic cells. The cells, both the pig Embryonic Germ cells (pEGCs) and the pig Fetal Fibroblasts (pFF) show properties of pluripotency and self renewal.
