Project description:Single-nucleus RNA sequencing of positional airway organoids derived from human fetal lung at 4 anatomic regions (trachea, bronchus, NCA, distal)
Project description:We derived primary, epithelial-enriched organoids from 120 PCD human fetal lung, specifically from trachea, bronchus, non-cartilaginous airway, and distal airway. These positional airway organoids were grown in FANY airway media for the duration of 2 passages before collection for single-nucleus RNA sequencing (n = 1 biological replicate).
Project description:We performed micro-dissections of developing human lungs during the late pseudoglandular stage and performed single nuclear RNA-sequencing (n = 4; biological replicates from 103-122 PCD) on trachea, bronchi, non-cartilaginous airways, and distal lung.
Project description:We performed micro-dissections of developing human lungs during the late pseudoglandular stage and performed single nuclear ATAC-sequencing (n = 2; biological replicates from 103-122 PCD) on trachea, bronchi, non-cartilaginous airways, and distal lung.
Project description:We performed micro-dissections of developing human lungs during the late pseudoglandular stage and performed single nuclear RNA-sequencing (n = 4; biological replicates from 103-122 PCD) on trachea, bronchi, non-cartilaginous airways, and distal lung.