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JaDipPall

ABSTRACT: Dipsastraea pallida

PROVIDER: PRJEB88291 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
		Other
	jaDipPall1.Bacteroidia_1.1.primary.fa.gz	Other
	jaDipPall1.Hoeflea_sp_1.1.primary.fa.gz	Other
	jaDipPall1.Mastigocoleus_sp_1.1.primary.fa.gz	Other
	jaDipPall1.metagenome.1.primary.fa.gz	Other

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jaDipPall

Project description:Dipsastraea pallida, genomic and transcriptomic data

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DNAseq of nine Seresta-selected and unselected Globodera pallida populations

Project description:DNA sequencing of nine Globodera pallida populations selected either for four generations on the resistant potato variety Seresta (S4), or, as a control, for one generation on the susceptible variety Desiree (D). Seresta contains GpaV from Solanum vernei as a major resistance against G. pallida. These nine populations were obtained from Dutch potato fields, where they previous to the further selection already showed some degree of virulence on resistant potato varieties. The goal of the experiment was to identify polymorphic loci in the genome associated with the selection on Seresta.

2026-03-16 | E-MTAB-16285 | biostudies-arrayexpress

DNAseq of five generations of a selection experiment on two populations of Globodera pallida

Project description:We aimed to identify loci under selection for virulence in the potato cyst nematode Globodera pallida. Therefore, two G. pallida populations with suspected virulence, designated AMPOP02 and AMPOP10, were used in a selection experiment on the resistant potato variety Seresta. The selection experiment started in 2015, going through one generation per year, leading to fourth-generation selection populations at the end of 2018. The experiment was conducted in large pots, with a population that was as large as possible. After each generation cysts were saved for a later pot-experiment. This pot experiment was conducted in 2019 where the replication of these G. pallida populations on a set of potato cultivars (amongst others the non-resistant cultivar Desiree and the resistant cultivar Seresta). The first priority with the resulting generation was quantification and thereafter the material resulting from the Desiree and Seresta propagation was frozen in liquid nitrogen for DNA isolation. For each generation/potato cultivar combination three replicates were conducted. In total we had therefore 60 samples. Unfortunately, after DNA isolation, we had material of sufficient quantity and quality to conduct DNA sequencing on 45 samples.

2025-08-05 | E-MTAB-15408 | biostudies-arrayexpress

Dipsastraea veroni

Project description:Dipsastraea veroni symbionts Raw sequence reads

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Transcriptome and gene expression analysis during flower blooming in Rosa chinensis ‘Pallida’

Project description:Rosa chinensis ‘Pallida’ (Rosa L.) is one of the most important ancient rose cultivars originating from China. It contributed the ‘tea scent’ trait to modern roses. However, little information is available on the gene regulatory networks involved in scent biosynthesis and metabolism in Rosa. In this study, the transcriptome of R. chinensis ‘Pallida’ petals at different developmental stages, from flower buds to senescent flowers, was investigated using Illumina sequencing technology. De novo assembly generated 89,614 clusters with an average length of 428 bp. Based on sequence similarity search with known proteins, 62.9% of total clusters were annotated. Out of these annotated transcripts, 25,705 and 37,159 sequences were assigned to gene ontology and clusters of orthologous groups, respectively. The dataset provides information on transcripts putatively associated with known scent metabolic pathways. Digital gene expression (DGE) was obtained using RNA samples from flower bud, open flower and senescent flower stages. Comparative DGE and quantitative real time PCR permitted the identification of five transcripts encoding proteins putatively associated with scent biosynthesis in roses. The study provides a foundation for scent-related genes discovery in roses.

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Small RNA-seq on a time series of potato infected with Globodera pallida

Project description:We explored the sex determination of Globodera pallida. To do so, we grew cuttings of the potato cultivar Desiree on Gamborg B5 medium supplemented with either 1.5 g/L or 20 g/L sucrose. 14-day-old cuttings were inoculated with 100 G. pallida E400 Rookmaker juveniles. Infected root tissue was harvested at 1, 3, 6, and 9 dpi. Also, a subsample of the ppJ2 was kept for each batch. The experiment contains four time-separated batches. Small RNA sequencing was performed by BGI Hong Kong.

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Transcriptome sequencing on a time series of potato infected with Globodera pallida to explore the sex determination

Project description:We explored the sex determination of Globodera pallida. To do so, we grew cuttings of the potato cultivar Desiree on Gamborg B5 medium supplemented with either 1.5 g/L or 20 g/L sucrose. 14-day-old cuttings were inoculated with 100 G. pallida E400 Rookmaker juveniles. Infected root tissue was harvested at 1, 3, 6, and 9 dpi. Also, a subsample of the ppJ2 was kept for each batch. The experiment contains four time-separated batches. Transcriptome sequencing was performed by BGI Hong Kong. Two samples (B_4.2_1,5; B_4.3_1,5) were not taken along for sequencing because of a failed library prep.

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