Project description:The proteomic content of the extracellular vesicles (EVs) released by the liver fluke Opisthorchis viverrini has been addressed in the past, but they have focused on the small population pelleting at 120k vesicles (also named exosomes). Here we provide the first proteomic analysis of vesicles pelleting at 15k (microvesicles) using LC-MS/MS.

2024-09-20 | PXD020356 | Pride

Proteomic analysis of the 120k extracellular vesicles from Opisthorchis viverrini

Project description:The proteomic content of the extracellular vesicles (EVs) released by the liver fluke Opisthorchis viverrini has been addressed in the past. Here we employ a more comprehensive purification method of the 120k subpopulation of EVs and analyze proteins present in different locations of these EVs (including external trypsin-liberated peptides, cargo proteins and membrane proteins) using LC-MS/MS.

2022-03-15 | PXD020345 | Pride

Proteomics Identification of Opisthorchis viverrini Antigens in Excretory-Secretory matrix

Project description:Opisthorchis viverrini (Ov), a Group I biological carcinogen classified by the WHO, is a leading cause of cholangiocarcinoma (CCA) in the Greater Mekong Subregion. Transmission occurs via consumption of raw or undercooked fish harboring Ov metacercariae, which mature into adult Ov worms in the biliary tract, triggering chronic inflammation and malignant transformation. Despite the widespread use of Ov-antigen rapid test kits (Ov-ATKs) for non-invasive screening, current diagnostics are limited by cross-reactivity and dependence on crude Ov antigens. In this study, we employed a label-free proteomics approach to comprehensively analyze infection-associated stages of Ov (metacercariae and adult worms) to compare with urinary sample from Ov-infected and CCA patients. A total of 17,287 proteins were identified, 2,768 protein shares across group of samples and 3,977 proteins were consistently upregulated in Ov-positive urine samples, we provide the first direct evidence that Ov-derived proteins are present in the urine of infected individuals, confirming systemic dissemination from the parasite. We also identified key protein signatures associated with both acute and chronic infection. These findings offer key insights into parasite specific protein and support the development of recombinant antigen-based diagnostics, enhancing Ov-ATKs specificity and enabling more accurate, scalable screening in endemic areas.

2026-02-26 | PXD073886 | Pride

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