Project description:Phosphorus (P) are pivotal element for proper plant growth and development. We performed microarray analysis of rice root under phosphorus deficiency (-P) to obtain a global view of gene regulations associated with plant response to -P.
Project description:Nitrogen (N) and phosphorus (P) are pivotal element for proper plant growth and development. We performed microarray analysis of rice shoot and root after nitrogen deficiency (-N) treatment under phosphorus deficiency (-P) condition to obtain a global view of gene regulations associated with plant response to -N under -P condition.
Project description:Root transcriptomes of acidic soil adapted rice genotypes viz. Sahbhagi Dhan (SD) and Chakhao Poreiton (CP) was done in response to low phosphorus (P) levels. RNAseq approach after 15 days of low P treatment was employed to understand long term molecular processes involved in low P tolerance. Note: Samples in SRA were assigned the same sample accession. This is incorrect as there are different samples, hence âSource Nameâ was replaced with new values. Comment[ENA_SAMPLE] contains the original SRA sample accessions.
Project description:Macronutrients are pivotal elements for proper plant growth and development. We performed microarray analysis of rice root under nitrogen (N), phosphorus (P), and potassium (K) deficiency conditions to obtain a global view of gene regulations associated with plant response to essential nutrients.
Project description:Macronutrients are pivotal elements for proper plant growth and development. We performed microarray analysis of rice shoot under nitrogen (N), phosphorus (P), and potassium (K) deficiency conditions to obtain a global view of gene regulations associated with plant response to essential nutrients.
Project description:Masson pine (Pinus massoniana) has evolved some adaptations for growth in low P soils. To elucidate these mechanisms, we investigated global gene expression profiles of the masson pine responding to long-term phosphorus starvation and different Pi levels (P1, 0.01 mM P; P2, 0.06 mM P). Analysis used phosphorus-sufficient treatment RNA as control samples for comparison to the experimental samples (P1 and P2) taken at 12, 24, 48 and 60 day. Indirect comparisons were made across multiple arrays with raw data pulled from different channels for data analysis and comparison to the control data.
Project description:Carbon (C), nitrogen (N) and phosphorus (Pi) are crucial macronutrients for plants. While substantial knowledge exists on C/N balance signaling physiological and molecular perspectives, the core regulatory mechanism governing C/N and Pi balance remains largely unexplored. This study reveals that C/N assimilatory metabolisms matching the Pi-deficiency stress is integrated by a DEEP GREEN PANICLE1 (DGP1)-mediated regulatory network in rice (Oryza sativa). We found that low-Pi stress triggers DGP1 expression in a dose-responsive manner, facilitated by the PHOSPHATE STARVATION RESPONSE REGULATOR (PHR2). Intriguingly, DGP1 interacts with transcription factors (GLK1 and GLK2) and mitochondrial factors (GDCH and QCR9), modulating their roles in photosynthetic gene expression, the glycine cleavage system and the mitochondrial electron transport chain (mETC), respectively. Additionally, DGP1 influences aminoacyl-tRNA synthetases and ribosomal subunits, impacting protein biosynthesis, and intervenes in various metabolic pathways, including glycolysis and the citrate cycle. Phenotypically, we observed that under short-term Pi-deficiency, wild-type plants exhibit compromised photosynthesis, photorespiration and mETC-driven ATP synthesis, whereas dgp1 and phr2 mutants display reduced sensitivity to these conditions. Conversely, DGP1 overexpressors show decreased yield under normal conditions due to altered cell metabolomes and protein biosynthesis inadequacy, despite unaffected 15NO3– uptake and transport. In conclusion, the PHR2-DGP1 module orchestrates a comprehensive signaling network, harmonizing C and N metabolic fluxes under Pi limitation through coordinated interactions among cytoplast, chloroplast and mitochondria.
Project description:Masson pine (Pinus massoniana) has evolved some adaptations for growth in low P soils. To elucidate these mechanisms, we investigated global gene expression profiles of the masson pine responding to long-term phosphorus starvation and different Pi levels (P1, 0.01 mM P; P2, 0.06 mM P).
Project description:This study aims to deal with a comparative proteome analysis of shoot and root tissues of contrasting phosphorus (P) responsive rice genotypes (Pusa-44 and its near-isogenic line (NIL)-23 harboring Pup1 QTL). Proteins were isolated from shoot and root tissues collected from 45-day-old rice plants grown hydroponically in PusaRicH medium with P (16ppm, +P) or without P (0 ppm, -P) under controlled environmental conditions. Following protein quantification using the Bradford method, and quality check using 1D SDS-PAGE, trypsin was used for in-solution digestion and Nano ACQUITY UPLC-MS/MS was used to separate and identify peptides. This study aimed at deciphering the molecular aspect of Pup1 QTL in P-starvation stress tolerance in rice.
