Project description:The degradation of glycosaminoglycans (GAGs) by intestinal bacteria is critical for their colonization in the human gut and the health of the host. Both Bacteroides and Firmicutes have been reported to degrade GAGs, while the enzymatic details of the latter remain largely unknown. In this study, we isolated a Firmicutes strain, Hungatella hathewayi N2-326, that can catabolize various GAGs. While H. hathewayi N2-326 was less efficient in utilizing chondroitin sulfate A (CSA) and dermatan sulfate (DS) than Bacteroides thetaiotaomicron, a characterized GAG degrader, it outperformed B. thetaiotaomicron in assimilating hyaluronic acid. Unlike B. thetaiotaomicron, H. hathewayi N2-326 could not utilize heparin. The chondroitin lyase activity of H. hathewayi N2-326 was found to be induced by CSA and displayed both cell-associated and extracellular distributions. We further identified and characterized the first chondroitin ABC lyase from Firmicutes. The recombinant H. hathewayi chondroitin ABC lyase was found to be a predominantly exolyase and exhibited higher specific activity than any other characterized chondroitin ABC lyase. Thus, the HH-chondroitin ABC lyase offers a viable commercial option for the production of chondroitin, dermatan, and hyaluronan oligosaccharides and potential medical applications.

2022-08-14 | GSE211011 | GEO

Machine Learning-Enabled Pipeline for Large-Scale Virtual Drug Screening.

Project description: Not available

| S-EPMC8478848 | biostudies-literature

Large-scale sequence and structural comparisons of human naive and antigen-experienced antibody repertoires.

Project description: Not available

| S-EPMC4868480 | biostudies-literature

Large-scale genome-wide analyses of stuttering.

Project description: Not available

| S-EPMC12339392 | biostudies-literature

Understanding Dyslexia Through Personalized Large-Scale Computational Models.

Project description: Not available