Project description:Group 3 medulloblastoma is the most aggressive among the four medulloblastoma subgroups, with poor prognosis and early metastasis making treatment particularly challenging. The lack of an immune-competent mouse model has hindered effective in vivo testing of potential drugs and mechanisms. Given the prevalent MYC overexpression in Group 3 medulloblastoma, we developed a Group 3-like medulloblastoma mouse model in C57BL/6J mice by overexpressing MYC in the cerebellum of newborn pups. This model provides a valuable tool for studying Group 3 medulloblastoma more effectively.
Project description:Group 3 medulloblastoma is the most aggressive among the four medulloblastoma subgroups, with poor prognosis and early metastasis making treatment particularly challenging. The lack of an immune-competent mouse model has hindered effective in vivo testing of potential drugs and mechanisms. Given the prevalent MYC overexpression in Group 3 medulloblastoma, we developed a Group 3-like medulloblastoma mouse model in C57BL/6J mice by overexpressing MYC in the cerebellum of newborn pups. This model provides a valuable tool for studying Group 3 medulloblastoma more effectively.
Project description:PRTG+ve cells show high self renewability in Group 3 medulloblastoma tumors. To access the proteins differentially expressed in this subset of cells, we sorted PRTG+ve and PRTG-ve cells by surface staining with Anti-PRTG antibody from Gr3 medulloblastoma xenografts.
Project description:Investigate the DNA binding pattern as well as transcriptional consequences of ZIC1 and its medulloblastoma mutants in group 3 medulloblastoma cell lines and granule neuron progenitors
Project description:Investigate the DNA binding pattern as well as transcriptional consequences of ZIC1 and its medulloblastoma mutants in group 3 medulloblastoma cell lines and granule neuron progenitors
Project description:We evaluated changes in active and repressive histone modifications following the silencing of OTX2 in Group 3 medulloblastoma tumorspheres
Project description:Retina-specific gene expression is the distinguishing characteristic of Group 3 medulloblastoma. CRX, a homeobox transcription factor, is overexpressed specifically in Group 3 tumors. ShRNA-mediated CRX knockdown decreased the expression of seveal retina-specific genes and Group 3 specific genes. CRX knockdown inhibited the TGF-beta/activin signaling pathway, which is known to play oncogenic role in a subset of Group 3 medulloblastoma