Project description:PRTG+ve cells show high self renewability in Group 3 medulloblastoma tumors. To access the proteins differentially expressed in this subset of cells, we sorted PRTG+ve and PRTG-ve cells by surface staining with Anti-PRTG antibody from Gr3 medulloblastoma xenografts.
Project description:Investigate the DNA binding pattern as well as transcriptional consequences of ZIC1 and its medulloblastoma mutants in group 3 medulloblastoma cell lines and granule neuron progenitors
Project description:Investigate the DNA binding pattern as well as transcriptional consequences of ZIC1 and its medulloblastoma mutants in group 3 medulloblastoma cell lines and granule neuron progenitors
Project description:We evaluated changes in active and repressive histone modifications following the silencing of OTX2 in Group 3 medulloblastoma tumorspheres
Project description:Retina-specific gene expression is the distinguishing characteristic of Group 3 medulloblastoma. CRX, a homeobox transcription factor, is overexpressed specifically in Group 3 tumors. ShRNA-mediated CRX knockdown decreased the expression of seveal retina-specific genes and Group 3 specific genes. CRX knockdown inhibited the TGF-beta/activin signaling pathway, which is known to play oncogenic role in a subset of Group 3 medulloblastoma
Project description:Transcripts bound to Musashi-1 (MSI1) are explored in Group 3 medulloblastoma (G3 MB) and neural stem cells harvested from the cerebellar portion of a embryonal brain
Project description:These data were generated in May of 2016 on a Thermo Q-Exactive at the University of Cape Town in South Africa. Samples were drawn from 48 cases of malignant medulloblastoma that were added to the FFPE archive between 1988 and 2014 under approval from the University of Cape Town Faculty of Health Sciences Human Research Ethics Committee (HREC 149/2014). Assignment to Group 3 or Group 4 categories was performed via Nanostring analysis and IHC (other subtypes were also analyzed in the larger study). Five distinct samples from each of the two Groups were subjected to LC-MS/MS. After conversion to mzML, a total of 245,589 tandem mass spectra were available, of which 36% corresponded to doubly-charged precursor ions. Over all ten experiments, tandem mass spectra were acquired at a rate of 4.55 Hz. MS/MS scans averaged a peak density average of 148 peaks per spectrum.
More complete information is available in Omesan Nair's Ph.D. Dissertation: http://hdl.handle.net/11427/26896.