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Transcriptome profiling of myeloid cell lines in Gelatin Methacrylate Hydrogels

ABSTRACT: Transcriptome profiling of myeloid cell lines in Gelatin Methacrylate Hydrogels

PROVIDER: PRJEB93879 | ENA |

REPOSITORIES: ENA

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Polyvinyl alcohol/gelatin hydrogels regulate cell adhesion and chromatin accessibility

Project description:Cell adhesion is known to heavily influence cell migration and wound healing. A number of cell adhesion molecules and their roles has been extensively studied. However, the changes of global chromatin accessibility and gene expressions correlated with cell adhesion is still unclear. Here we prepared a series of PVA/Gelatin hydrogels to handily regulate cell adhesion by adjusting gelatin concentration and found that cell adhesion area and the ratio of non-circular cells were increased with the increasement of gelatin concentration while cell circularity was decreased. Our results showed that widespread chromatin accessibility was increased with increasing expression level of histone deacetylase 11 (Hdc11) under poor cell adhesion. Meanwhile, one branch of MAPK pathway - ERK 1/2 which involved in mechanotransduction signaling was also found in the enrichment analysis of downregulated genes. This work provided a handy method to regulate cell adhesion and these findings deepen our understanding of the cell adhesion process and its associated chromatin accessibility.

2022-08-11 | GSE190091 | GEO

Culturing C2C12 myotubes on micromolded gelatin hydrogels accelerates myotube maturation

Project description:Culturing C2C12 myotubes on micromolded gelatin hydrogels accelerates myotube maturation

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Polyvinyl alcohol/gelatin hydrogels regulate cell adhesion and chromatin accessibility

Project description:Polyvinyl alcohol/gelatin hydrogels regulate cell adhesion and chromatin accessibility

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Single cell analysis of iPSC-derived cortical organoids in biofunctionalized gelatin hydrogels

Project description:Single cell analysis of iPSC-derived cortical organoids in biofunctionalized gelatin hydrogels

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Single cell analysis of iPSC-derived cortical organoids in biofunctionalized gelatin hydrogels

Project description:Single cell analysis of iPSC-derived cortical organoids in biofunctionalized gelatin hydrogels

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Culturing C2C12 myotubes on micromolded gelatin hydrogels accelerates myotube maturation

Project description:We report that differentiation of the C2C12 myoblast line on patterened hydrogels results in the increased expression of muscle sarcomere genes and more closely models expression changes that happen in in vivo muscle differentiation than do C2C12 myoblasts cultured on unpatterened substrates.

2020-04-15 | GSE148667 | GEO

Single cell analysis of iPSC-derived cortical organoids in biofunctionalized gelatin hydrogels

Project description:Human neural organoid models have become an important tool for studying neurobiology. In this work, we compared Matrigel to an N-cadherin peptide-functionalized gelatin methacryloyl hydrogel (termed GelMA-Cad) for culturing cortical neural organoids. Specifically, we compare five materials: (1) Matrigel, (2) GelMA-Cad with high crosslinker (HC), (3) GelMA-Cad with low crosslinker (LC), (4) GelMA HC and (5) GelMA LC. We determined that both mechanical properties and peptide presentation can tune cell fate and diversity in gelatin-based matrices during differentiation. Of particular note, cortical organoids cultured in GelMA-Cad produce higher numbers of neurogenic and ciliated radial glia and upper-layer excitatory neurons—an important population for modeling neurodegenerative disease—compared to GelMA and Matrigel controls.

2024-02-28 | E-MTAB-13828 | biostudies-arrayexpress

Single cell analysis of iPSC-derived cortical organoids in biofunctionalized gelatin hydrogels

2024-07-01 | E-MTAB-14203 | biostudies-arrayexpress

Transcriptome analysis of the differentiated C2C12 cells in 3 different substrate, Tissue culture plate, Methacarylated gelatin and 15% Methacarylated gelatin mixed with 1% PSS (poly sodium 4-styrenesulfonate)

Project description:Purpose : To determine transcriptome profile of the diffferentiated C2C12 cells in 3 different cell culture substrate, Tissue culture plate, Methacarylated gelatin and 15% Methacarylated gelatin mixed with 1% PSS (poly sodium 4-styrenesulfonate) Method : RNA smples prepared from differentiated C2C12 cells in 3 different cell culture substrate, Tissue culture plate, Methacarylated gelatin and 15% Methacarylated gelatin mixed with 1% PSS (poly sodium 4-styrenesulfonate) Results : Paired-ends 101bp reads, we mapped about 72 million reads - 145 million reads per sample to the mouse genome (build mm10) and assembled with cufflinks program (value : FPKM)

2021-01-26 | GSE165480 | GEO

RNA-seq analysis of mESCs cultured on either Laminin-511 or gelatin coated dishes in either Std or 2i medium

Project description:Some mouse embryonic stem cell (mESC) lines need to be maintained on feeder cells in gelatin/Std condition. To eliminate the need for feeder cells, we decided to maintain the C57BL6/J mESCs on dishes coated with Laminin-511 (LN511) enabling maintenance of mESCs without feeder cells in Std condition (Domogatskaya et al., 2008). To compare the transcriptomes of mESCs cultured on gelatin-coated dish and those cultured on Laminin-511 coated dish, we performed RNA-Seq analysis. We found that the transcriptomes of mESCs cultured on gelatin-coated dish and those cultured on LN511 coated dish showed no considerable difference in expression patterns.

2020-06-04 | GSE132591 | GEO

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