Project description:Metagenome data from soil samples were collected at 0 to 10cm deep from 2 avocado orchards in Channybearup, Western Australia, in 2024. Amplicon sequence variant (ASV) tables were constructed based on the DADA2 pipeline with default parameters.
Project description:To examine variability in differentiation outcomes, distinct iPCS lines were differentiated to intermediate mesoderm following the protocol of Takasato et al (2015). Cells were treated with 4 or 5 days of CHIR followed by 4 days of FGF9, dissociated and profiled to generate bulk transcriptome data for each. Cell lines used were "MAFB-mtagBFP;GATA3-mCherry" described in Vanslambrouck et al, JASN, 2019; "NG-hiPSC line1B", D2MCBL01B, described in Novoa et al, Cytotherapy 2024; AICS-0061, "H2B-GFP AICS", a H2B-GFP reporter from the Allen Institute of Cell Science, Allen Cell Collection.
