Project description:Biomechanical alterations of the extracellular matrix (ECM) and injury-induced or disease-induced cell loss are major contributors to pathological astrocyte activation (astrogliosis) in the CNS and the formation of glial scars. This in vitro experiment is intended to identify transcriptional alterations in mixed glial cultures grown on surfaces of different stiffness (rigid, 650 Pa hydrogel, 250 Pa hydrogel) and also in a confluent mixed glial monolayer on a stiff surface after injury by scratching with a plastic pipette tip.100,000 mixed glial cells were plated on different growth surfaces. After adhesion, confluent monolayers on different hyaluoronic acid/gelatin rigid or hydrogel surfaces were grown in low serum (0.5 % FBS) media for 48 h before RNA extraction. Adherent confluent monolayers on rigid PDL-coated surfaces were left uninjured or scratch-injured and were grown in low serum (0.5 % FBS) media for 48 h before RNA extraction.

2026-06-29 | E-MTAB-17287 | biostudies-arrayexpress

cbBryCale10

Project description:Bryoerythrophyllum caledonicum (Scottish beardmoss) genome assembly, cbBryCale10

| PRJEB73528 | ENA

TGF-beta-mediated microRNA expression in breast cancer cells

Project description:We utilized 3D-organotypic cultures whose physical properties were altered by inclusiong of type I collagen to create biomechanically rigid microenvironments that approximated those typically observed in primary mammary tumors. Compliant 3D-organotypic cultures were also generated to recapitulate the biomechanical properties of pulmonary microenvironments typically encountered by disseminated breast carcioma cells. The murine 4T1 progression series represents an established model of triple negative breast cancer development and metastasis and consists of isogenically-derived nonmetastatic 67NR, systemically invasive 4TO7, and highly metastatic 4T1 cells that were propagated for 6 days in the absense or presense of TGF-beta in either rigid or compliant 3D-cultures. Afterward, total RNA was extracted and subjected to miRNA profiling.

2012-10-31 | GSE41274 | GEO

cbBryCale

Project description:Bryoerythrophyllum caledonicum (Scottish beardmoss), genomic and transcriptomic data

| PRJEB70979 | ENA