Project description:To understand the role of WRKY10 transcription factor in foliar pigmentation patterns in Marchantia (liverwort) and assess the conservation of its regulatory network in Lactuca sativa var. Redfire(angiosperm).
Project description:To understand a role of MpMET in maintenance of cytpsine methylation and transposon silencing, we obtained a genome wide profile of 5-methylated cytosine from the Mpmet mutant of a model liverwort, Marchantia polymorpha.
Project description:Most of our current knowledge about the molecular events ruling plant-virus interaction come from studies focusing on vascular plants. We here characterized the molecular, cellular and physiological events goberning plant-virus interactions in the non-vascular liverwort Marchantia polymorpha.
Project description:We used RNA-seq to obtain a time course transcriptomics response on the liverwort Marchantia polymorpha as gemmae are released from dormancy and initiate germination. Dormant gemmae were collected from gemmae cups of adult thalli and subsequently subjected to different light treatments. Time point 0 was sampled without imbibition. All other samples were placed directly in 100 µl water for imbibition, keeping them wet throughout the sampling period. Imbibition for 1 to 12 hours was performed in constant light or constant darkness at 20°C. The imbibition process was interrupted by freezing the samples in liquid nitrogen after 1, 4, 8, and 12 hours. RNA-seq libraries were sequenced on single-read mode (101 bp) on an Illumina NovaSeq 6000 platform. There are two libraries per sample.