Project description:RNA-Seq technique was applied to investigate the effects of two semen collection methods (Pelleted vs Liquefied) and two sperm purification methods (SCLB vs PS) to the integrity of isolated RNAs at different perspectives. The same set of semen samples were applied to investigate the qualitative and quantitative effect of semen collection methods and sperm cell purification methods on sperm transcript profiling.

2013-12-09 | E-GEOD-43586 | biostudies-arrayexpress

Evaluation of the effectiveness of semen collection and sperm purification methods for spermatozoa transcript profiling

2013-12-09 | GSE43586 | GEO

bull metagenome

Project description:bull metagenome

| PRJNA438952 | ENA

Identification of Bull Semen Microbiome by 16S Sequencing and Possible Relationships with Fertility.

Project description:Reports on the use of 16S sequencing for the identification of bacteria in healthy animals are lacking. Bacterial contamination of bull semen can have a negative effect on the sperm quality. The aims of this study were threefold: to identify bacteria in the semen of healthy bulls using 16S sequencing; to investigate the differences in the bacterial community between individual bulls; and to establish if there was a relationship between the bacteria isolated and bull fertility. Semen from 18 bulls of known fertility was used for the DNA extraction and 16S sequencing; 107 bacterial genera were identified. The differences in the amplicon sequence variants (ASVs) and the numbers of genera between bulls were noted. Negative correlations (p < 0.05) between several bacterial genera with Curvibacter, Rikenellaceae RC9-gut-group and Dyella spp. were seen. Other negatively correlated bacteria were Cutibacterium, Ruminococcaceae UCG-005, Ruminococcaceae UCG-010 and Staphylococcus, all within the top 20 genera. Two genera, W5053 and Lawsonella, were enriched in bulls of low fertility; this is the first time that these bacteria have been reported in bull semen samples. The majority of the bacteria were environmental organisms or were species originating from the mucous membranes of animals and humans. The results of this study indicate that differences in the seminal microbiota of healthy bulls occur and might be correlated with fertility.

| S-EPMC8705814 | biostudies-literature

Bull sperm sncRNAs of the Montbeliarde breed

Project description:Bull sperm sncRNAs of the Montbeliarde breed

| PRJEB50561 | ENA

Whole geneome methylation sequencing for bull fertility DNA mehylation biomarker

Project description:High fertility and low fertility bulls were screened from the a 6000 bull database and then identify the sperm-derived DMR and DMC that assoiated with bull ferrtility via whole genome methtlation sequencing

2024-06-04 | GSE211794 | GEO

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