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Composition of the mucosa-associated microbiota along the entire gastrointestinal tract of human individuals

ABSTRACT: Microbiota along gastrointestinal tract of humans

PROVIDER: PRJEB96923 | ENA |

REPOSITORIES: ENA

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Composition of the mucosa-associated microbiota along the entire gastrointestinal tract of human individuals.

Project description:BackgroundHomeostasis of the gastrointestinal tract depends on a healthy bacterial microbiota, with alterations in microbiota composition suggested to contribute to diseases. To unravel bacterial contribution to disease pathology, a thorough understanding of the microbiota of the complete gastrointestinal tract is essential. To date, most microbial analyses have either focused on faecal samples, or on the microbial constitution of one gastrointestinal location instead of different locations within one individual.ObjectiveWe aimed to analyse the mucosal microbiome along the entire gastrointestinal tract within the same individuals.MethodsMucosal biopsies were taken from nine different sites in 14 individuals undergoing antegrade and subsequent retrograde double-balloon enteroscopy. The bacterial composition was characterised using 16 S rRNA sequencing with Illumina Miseq.ResultsAt double-balloon enteroscopy, one individual had a caecal adenocarcinoma and one individual had Peutz-Jeghers polyps. The composition of the microbiota distinctively changed along the gastrointestinal tract with larger bacterial load, diversity and abundance of Firmicutes and Bacteroidetes in the lower gastrointestinal tract than the upper gastrointestinal tract, which was predominated by Proteobacteria and Firmicutes.ConclusionsWe show that gastrointestinal location is a larger determinant of mucosal microbial diversity than inter-person differences. These data provide a baseline for further studies investigating gastrointestinal microbiota-related disease.

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Protein turnover rate along the gastrointestinal tract is coordinated by the spatial location and the microbiota

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